Liquid Biopsy
A liquid biopsy is only as sensitive as the weakest step in the workflow. ctDNA can make up less than 0.1% of total cfDNA in early-stage cancer — which means a suboptimal extraction, a PCR duplication artifact, or a library prep that loses short fragments can cost you the signal you were looking for.
This page brings together FireGene products validated for liquid biopsy workflows, organized by where they sit in the pre-analytical and library preparation pipeline.
cfDNA Extraction Two column-based options for plasma cfDNA isolation from 1–4 mL plasma input. The Plasma cfDNA Extraction Kit and Circulating Free DNA Extraction Kit are optimized for the short, highly fragmented nature of cfDNA — the 160–200 bp mononucleosomal fragments that standard genomic DNA extraction protocols systematically lose. Both deliver inhibitor-free, high-purity cfDNA compatible with ddPCR, targeted NGS panels, and whole-genome approaches.
PCR-Free Library Preparation The Non-Amplification DNA Library Kit for Illumina skips PCR amplification entirely — the step that introduces the duplication bias that distorts variant allele frequency measurements at low ctDNA concentrations. For cfDNA inputs where every molecule counts, removing PCR preserves the true representation of the circulating nucleic acid pool.
DNA Methylation Sequencing The Post-Bisulfite DNA Library Preparation Kit supports whole-genome bisulfite sequencing (WGBS) and reduced-representation methylation profiling from cfDNA — the foundation of methylation-based liquid biopsy approaches for tissue-of-origin classification and early cancer detection.
For complete workflow guidance, contact us directly.
