Tumor Single-Cell Sequencing Sample Prep
Tumors are among the hardest tissues to dissociate cleanly. Dense fibrotic stroma, necrotic regions, high immune infiltration, and a mix of fragile cell populations — each of these pushes generic dissociation protocols toward one of two failure modes: under-digestion that leaves cell aggregates, or over-digestion that strips surface markers, activates stress responses, and makes your immune clusters look like artifacts.
This collection covers two tumor-specific dissociation kits, matched to different levels of tissue complexity.
Tumor Dissociation Kit (FG-BA3320) Enzymatic kit optimized for dissociation of tumor tissue into high-viability single-cell suspensions for scRNA-seq and functional analysis. Designed to recover the diverse cell types that constitute the TME: malignant cells, stem-like cells, tumor-infiltrating immune cells, and stromal populations. The entry-point kit for solid tumor single-cell profiling — tumor heterogeneity, immune infiltration, treatment response mapping, and cell-cell interaction analysis.
Tumor Dissociation Kit Plus Designed for more complex tumor tissues. Uses enzymatic digestion with adjustable incubation time to accommodate variation in species, tissue mass, and necrotic content — the variables that cause a fixed-protocol kit to fail on samples from highly necrotic tumors or fibrotic tumor subtypes. Compatible with single-cell sequencing, cell culture, and functional assays.
Both kits are validated for 10x Genomics Chromium, BD Rhapsody, and Drop-seq workflows.
For surgical samples with same-day processing constraints or inter-site shipping, see the Sample Preservation Before Single-Cell Analysis collection.
