Description
FireGene Universal Plant Protoplast Isolation Kit is designed for the isolation and preparation of protoplast suspensions from higher plant tissues. The kit uses enzyme-based digestion to gently and effectively break down plant cell walls, releasing protoplasts into a single-protoplast suspension. The prepared protoplasts can be used for single-cell sequencing, tissue culture, protoplast fusion, and other plant cell research applications. The protocol includes enzymatic digestion, filtration, centrifugation, optional purification, and quality control by microscope or cell counter.
Background Information
Plant protoplasts are cells with the cell wall removed, making them useful for experiments that require direct access to the plasma membrane, cytoplasm, or cellular contents. Because plant cell walls can limit access during many experimental workflows, enzymatic protoplast isolation is widely used in plant biology, functional genomics, and cell engineering studies.
FireGene Universal Plant Protoplast Isolation Kit provides a practical workflow for preparing protoplast suspensions from fresh, tender plant tissue while supporting downstream applications that require viable and clean protoplast preparations.
Research Areas
This kit is suitable for plant research fields involving cellular function, gene regulation, tissue culture, and plant biotechnology.
- Plant cell biology
- Plant functional genomics
- Plant developmental biology
- Plant physiology
- Crop science research
- Plant stress response studies
- Plant tissue culture
- Plant genetic engineering
- Plant single-cell analysis
- Protoplast-based assay development
Key Applications
The prepared single-protoplast suspensions can be used in multiple downstream workflows.
- Plant single-cell sequencing
- Plant tissue culture
- Protoplast fusion
- Transient gene expression studies
- Plant cell transformation research
- Cell viability and morphology analysis
- Plant cell-type and heterogeneity studies
- Cell-based screening and functional assays
Specifications
| Specification | Details |
|---|---|
| Product Name | Universal Plant Protoplast Isolation Kit |
| Catalog No. | FG-BA3317 |
| Kit Size | 10 reactions |
| Sample Type | Higher plant tissue |
| Recommended Sample | Fresh, tender plant tissue |
| Recommended Starting Material | Approximately 200–300 mg tissue |
| Main Function | Isolation and preparation of plant protoplast suspensions |
| Workflow | Mannitol pretreatment, enzymatic digestion, filtration, centrifugation, optional PPS purification, protoplast resuspension |
| Downstream Applications | Single-cell sequencing, tissue culture, protoplast fusion, cell-related research |
| Required Instruments | Orbital shaker, surgical scalpel, horizontal centrifuge |
| Required Reagents | 15% mannitol solution, BSA |
| Required Consumables | Low-adsorption pipette tips, 30 mL conical flasks, 15 mL centrifuge tubes, 70 μm cell strainers |
| Digestion Conditions | 3–5 hours at 25°C or room temperature, with microscopic monitoring |
| Storage | Enzyme A: -20°C |
| Buffer Storage | Buffer B, PPS, and Buffer C: 4°C, protected from light |
| Shelf Life | Enzyme A: 2 years; Buffer B, PPS, and Buffer C: 1 year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| Enzyme A | FG-BA3317-A | 50 mL |
| Buffer B | FG-BA3317-B | 80 mL |
| PPS, Protoplast Purification Solution | FG-BA3317-C | 25 mL |
| Buffer C | FG-BA3317-G | 10 mL |