Cloning and Mutagenesis Kits
Restriction enzyme cloning works until the restriction site you need is already in your insert. Overlap extension PCR mutagenesis works until the fragment is too long and the error rate too high to trust the sequence. Both are still common in molecular biology labs — and both have a faster replacement that doesn't require gel purification between steps.
This collection covers two kits built around homologous recombination rather than ligation or annealing-based chemistry.
One Step Cloning Kit — seamless directional cloning Built on UvsXase, a recombinase derived from T4 phage. Directionally inserts PCR products into any linearized or restriction-digested vector at any site — restriction fragments and PCR-linearized vectors work directly, with no DNA purification required before the recombination reaction. Homology arms of 15–20 bp added to insert primers are sufficient for efficient directional cloning. Suitable for single-fragment insertion, multi-fragment assembly, and high-throughput cloning workflows.
Fast Site-Directed Mutagenesis Kit — whole-plasmid amplification and recombination Two-module system: a high-fidelity Max Master Mix amplification module for whole-plasmid PCR up to 20 kb, and a rapid homologous recombination module for circularization. DpnI digestion removes the original methylated template; the amplified product goes directly into the recombination reaction without gel purification. Introduces point mutations, insertions, or deletions at any position in plasmids under 20 kb in a single round of amplification.
