Description
FireGene Digestive Tissue Cell Debris Removal Kit is designed to remove dead cells, impurities, and cell debris from single-cell suspensions generated after dissociation of mammalian digestive system tissues. The workflow helps produce a cleaner background and lower debris content for downstream experiments such as flow cytometry analysis, single-cell sequencing, cell culture, and other cell-related assays. The protocol uses layered separation with DRS ① and DRS ②, followed by viable cell layer collection, washing, and quality control.
Background Information
Digestive system tissues can generate debris, impurities, mucus-associated material, and dead cells during dissociation. A dedicated cleanup workflow helps improve suspension clarity before downstream single-cell, flow cytometry, or culture-based analysis.
FireGene Digestive Tissue Cell Debris Removal Kit for Single-Cell Cleanup provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Gastrointestinal biology
- Digestive disease research
- Mucosal immunology
- Inflammation research
- Cancer and tumor microenvironment studies
- Single-cell biology
- Cell culture workflows
- Translational biomedical research
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Digestive tissue cell debris removal
- Single-cell suspension cleanup
- Flow cytometry analysis
- Single-cell sequencing preparation
- Cell culture workflows
- Cell viability and counting
- Post-dissociation sample processing
Specifications
| Product Name | Digestive System Tissue Cell Debris Removal Kit |
| Brand | FireGene |
| Catalog No. | FG-BA3329 |
| Kit Size | 10 reactions |
| Sample Type | Single-cell suspensions from mammalian digestive system tissues |
| Main Function | Removal of dead cells, impurities, and cell debris |
| Workflow | Centrifugation, DRS ① mixing, DRS ② layering, separation, viable cell layer collection, washing, resuspension |
| Downstream Applications | Flow cytometry analysis, single-cell sequencing, cell culture, cell-related assays |
| Separation Conditions | 4°C, 1400 ×g, 20 minutes |
| Required Instruments | Horizontal centrifuge, cell counter |
| Required Reagents | RPMI 1640 medium, PBS, FBS |
| Required Consumables | Low-adhesion pipette tips, centrifuge tubes, 20 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | 4°C, protected from light |
| Shelf Life | One year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| (DRS ①) Digestive System Tissue Cell Debris Removal Solution ① | FG-BA3329-A | 10 mL |
| (DRS ②) Digestive System Tissue Cell Debris Removal Solution ② | FG-BA3329-B | 20 mL |