FireGene Viral DNA/RNA Isolation Kit

Description

The FireGene's Viral DNA/RNA Isolation Kit is designed for the rapid coextraction of viral DNA and RNA from a variety of biofluid samples, such as, plasma, serum, milk and swab samples. The proprietary microspherical paramagnetic beads used in the kit have large binding surfaces and a high affinity towards nucleic acids.

From sample lysis/binding, washing, to the elution step, the whole process can be completed under 35 minutes, and yields highly pure nucleic acids elute.

The recovered nucleic acids can be used in a wide range of applications, such as PCR, RT-PCR, Sanger Sequencing, NGS, and gene chips.

It provides tremendous user benefits, such as, minimal manual operating steps, extremely low sample-to-sample variations, and high throughput. This Kit is capable of conducting 480 reactions.

Specifications

Product Type	Nucleic Acid Extraction/Purification
Applications	PCR, RT-PCR, Sanger Sequencing, NGS, and Gene Chips
Compatible Sample Types	Bio-fluid samples including serum, plasma, urine, cerebrospinal fluid, swab soaking fluid, culture medium supernatant and other samples
Target	Viral DNA and RNA
Method	Scalable, flexible format with magnetic beads
Prep Time	< 35 minutes
Amount of Starting Material
Supported Instruments	Compatible with most automated nucleic acid extraction instruments, high-throughput compatible
Number of Reactions	96
Storage	Proteinase K and magnetic beads should be stored in a refrigerator at 2–8°C; other components should be stored at room temperature.
Shelf-life	12 months

 

Kit Components

This product consists of lysis buffer (main components are sodium chloride, guanidine isothiocyanate, and Tris-HCl), washing buffer 1 (main components are sodium chloride and guanidine isothiocyanate), washing buffer 2 (main component is water), elution buffer (main component is water), magnetic beads (main component is magnetic beads), and proteinase K (main component is proteinase K).

Components Name	10 times	Storage temperature
Lysis Buffer	5mL	RT.
Proteinase K	250µL	2-8℃
Magnetic bead suspension	325µL	2-8℃
Wash buffer 1	3mL	RT.
Wash buffer 2	2.5mL	RT.
Elution solution	1.2mL	RT.