Description
Description
[Intended Use]
Used for nucleic acid extraction, enrichment, purification, etc.
The processed products are used for clinical in vitro detection.
The product components do not contain antigens, antibodies, probes or other components that can specifically bind to samples.
[Test Principle]
This reagent adopts a safe and non-toxic lysis buffer formula to rapidly release DNA from samples. Through a high-efficiency binding and purification system, DNA with good integrity and high purity can be extracted.
It is suitable for rapid isolation and purification of microbial genomic DNA from clinical human pathogenic microorganisms.
Using specially surface-treated, nano-scale, superparamagnetic microspheres to selectively adsorb nucleic acids, high-quality DNA extraction is achieved.
[Main Components]
This product is mainly composed of Grinding Beads, Buffer A, Buffer B, Buffer D, Wash 1, Wash 2, Elution Buffer, Magnetic Bead Suspension, and Proteinase K.
|
Component Name |
48 Tests |
|
Proteinase K (20 mg/mL) |
1 mL/tube |
|
Buffer A |
12 mL/bottle |
|
Buffer B |
12 mL/bottle |
|
Buffer D |
30 mL/bottle |
|
Wash 1 |
30 mL/bottle |
|
Wash 2 |
15 mL/bottle |
|
Elution Buffer |
6 mL/bottle |
|
Magnetic Bead Suspension |
2 mL/tube |
|
Grinding Beads |
48 tubes |
* Before use, add the indicated volume of absolute ethanol to Wash 2 as labeled on the bottle.
The product composition is the same for manual and automatic operation.
Specifications
| Product Type | Nucleic Acid Extraction |
| Applications |
Enzyme digestion, library construction, cloning, PCR, quantitative PCR, chip detection, and high-throughput sequencing |
| Compatible Sample Types | Microbial samples |
| Target | DNA |
| Method | Bacterial wall breaking - digestion and lysis - magnetic beads binding nucleic acid - washing to remove impurities - elution to obtain high quality nucleic acid |
| Prep Time | |
| Amount of Starting Material | |
| Supported Instruments | Compatible with most automated nucleic acid extraction instruments and pipetting workstations |
| Number of Reactions | 48 |
| Storage |
-20°C for Proteinase K 2~8°C for Magnetic Bead Suspensions Room temperature for all other components |
| Shelf-life | 12 months |
Product FAQ
1. Does this item contain any surfactant? If yes, please let me know its name and concentration.
Contains surfactant, namely 10% sodium lauryl sarcosinate and 1.3% Triton X-100
2. Does this contain these 3 preservatives? - Sodium azide -Thimerosal -2-mercaptoethanolIf yes, please let me know its concentration.
Does not contain these three substances
3. Does this contain ethanol?
If yes, please let me know its concentration.
The product contains 50% isopropyl alcohol and no ethanol (ethanol needs to be provided by the customer)
4. Please let me know the name and CAS# of the beads.
The beads are zirconia beads (No CAS number)
5. Is this used insect cells under manufacturing process?
Insect cells are not utilized in the production process
Components