Description
FireGene Moth Intestinal Dissociation Kit is designed for the isolation and preparation of cell suspensions from moth intestinal tissues. The kit uses enzymatic digestion to gently and effectively break down extracellular matrix and release cells. Prepared cell suspensions can be used for cell sorting, cell culture, and other cell-related assays. The workflow is optimized for insect tissue handling, with gentle pipetting and wide-bore tips recommended because insect cells are relatively fragile.
Background Information
Moth intestinal tissue is relevant to insect physiology, host-pathogen interaction, microbiome, and pest biology research. Because insect cells can be fragile, gentle processing conditions are important during dissociation and resuspension.
FireGene Moth Intestinal Dissociation Kit for Single-Cell Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Insect biology
- Moth intestinal physiology
- Host-pathogen interaction studies
- Insect microbiome research
- Pest biology and toxicology
- Cell culture assay development
- Cell sorting workflows
- Comparative cell biology
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Moth intestinal cell suspension preparation
- Cell sorting
- Cell culture
- Cell viability and counting
- Insect intestinal tissue analysis
- Cell-based assays
- Tissue-specific insect research
Specifications
| Product Name | Moth Intestinal Dissociation Kit |
| Brand | FireGene |
| Catalog No. | FG-BA3347 |
| Kit Size | 10 reactions |
| Sample Type | Moth intestinal tissue |
| Compatible Samples | Fresh intestinal tissues from moths |
| Recommended Starting Material | Intestinal tissues from 10–15 fresh moths |
| Main Function | Preparation of moth intestinal cell suspensions |
| Workflow | Enzymatic digestion, filtration, centrifugation, optional wash, resuspension |
| Downstream Applications | Cell sorting, cell culture, cell-related assays |
| Digestion Conditions | 28°C for 25–35 minutes |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | D-Hanks buffer, calcium and magnesium free; FBS |
| Required Consumables | Low-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 40 μm cell strainers |
| Storage | -20°C |
| Shelf Life | Two years |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| MIDS (Moth Intestinal Dissociation Solution) | FG-BA3347-A | 30 mL |