Description
FireGene Mouse Skin Dissociation Kit is designed for enzymatic dissociation of mouse skin tissue and preparation of single-cell suspensions. The workflow addresses common challenges in mouse skin processing, including difficult dissociation and reduced cell viability. Prepared cells can be used for single-cell sequencing, cell sorting, cell culture, and related cellular experiments. The protocol uses a two-step enzymatic digestion workflow with filtration, washing, resuspension, and quality control.
Background Information
Mouse skin tissue contains layered epithelial, stromal, vascular, and immune components that can make dissociation challenging. A tissue-specific enzymatic workflow helps prepare cell suspensions for downstream cellular analysis while allowing digestion time to be adjusted by sample condition.
FireGene Mouse Skin Dissociation Kit for Single-Cell Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Mouse skin biology
- Dermatology research
- Wound healing studies
- Skin inflammation and immunology
- Barrier tissue research
- Cancer and tumor microenvironment studies
- Single-cell biology
- Cell culture assay development
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Mouse skin single-cell suspension preparation
- Single-cell RNA sequencing
- Flow cytometry and cell sorting
- Primary skin cell culture
- Cell viability and counting
- Skin tissue heterogeneity analysis
- Inflammation and immune profiling
Specifications
| Product Name | Skin Dissociation Kit, mouse |
| Brand | FireGene |
| Catalog No. | FG-BA3340 |
| Kit Size | 10 reactions |
| Sample Type | Mouse skin tissue |
| Compatible Samples | Fresh mouse skin tissue |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Preparation of single-cell suspensions |
| Workflow | Two-step enzymatic digestion, filtration, centrifugation, washing, resuspension, quality control |
| Downstream Applications | Single-cell sequencing, cell sorting, cell culture |
| Digestion Conditions | 37°C; 30 minutes with MSDS 1, then approximately 0.5–1 hour with MSDS 2 |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | RPMI 1640 medium, PBS, FBS |
| Required Consumables | Low-adsorption pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | -20°C |
| Shelf Life | Two years |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| MSDS1 (Mouse Skin Dissociation Solution 1) | FG-BA3340-A | 30 mL |
| MSDS2 (Mouse Skin Dissociation Solution 2) | FG-BA3340-B | 3 mL |