Description
FireGene Muscle Dissociation Kit is designed for the isolation and preparation of single-cell suspensions from muscle tissue. The kit uses enzyme-based digestion to gently and efficiently disrupt extracellular matrix and release cells. Prepared cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays. The protocol uses approximately 200 mg of fresh tissue, digestion at 37°C, filtration through a 70 μm cell strainer, washing, resuspension, and quality control.
Background Information
Muscle tissue is dense and fibrous, which can make dissociation challenging. Enzymatic digestion provides a practical approach for preparing muscle-derived cell suspensions for downstream single-cell and culture-based research.
FireGene Muscle Dissociation Kit for Single-Cell Suspension Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Skeletal muscle biology
- Muscle development and regeneration
- Neuromuscular disease research
- Metabolic disease studies
- Exercise physiology
- Regenerative medicine
- Single-cell biology
- Drug discovery and screening
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Muscle tissue single-cell suspension preparation
- Single-cell sequencing
- Cell culture
- Cell viability and counting
- Cell-based assays
- Muscle tissue heterogeneity analysis
- Disease model research
Specifications
| Product Name | Muscle Dissociation Kit |
| Brand | FireGene |
| Catalog No. | FG-BA3355 |
| Kit Size | 10 reactions |
| Sample Type | Muscle tissue |
| Compatible Samples | Fresh muscle tissue |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Preparation of muscle single-cell suspensions |
| Workflow | Enzymatic digestion, filtration, centrifugation, washing, resuspension, quality control |
| Downstream Applications | Single-cell sequencing, cell culture, cell-related assays |
| Digestion Conditions | 37°C for 0.5–2 hours |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | PBS, FBS, RPMI 1640 culture medium |
| Required Consumables | Low-adsorption pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | -20°C |
| Shelf Life | Two years |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| MTDS (Muscle Tissue Dissociation Solution) | FG-BA3355-A | 2 × 1.25 mL |