Description
FireGene Serum-Free One-Step Cell Freezing Medium is designed for the cryopreservation of animal primary cells and cell lines, especially cells that require a clear culture background or are sensitive to animal-origin components and DMSO. The formulation is serum-free, animal-origin-free, antibiotic-free, non-toxic, and contains low DMSO. The workflow does not require programmed cooling; cells can be resuspended in the freezing medium, aliquoted into cryovials, and placed directly at -80°C before optional transfer to liquid nitrogen for longer-term storage.
Background Information
Cell freezing medium is used to protect cells during low-temperature storage and help preserve cell recovery after thawing. For many research workflows, serum-free and animal-origin-free formulations are preferred because they reduce undefined biological components and help maintain a clearer culture background.
FireGene Serum-Free One-Step Cell Freezing Medium is suitable for animal primary cells and cell lines that require simplified, serum-free cryopreservation without programmed cooling.
Research Areas
This freezing medium is suitable for laboratories working with primary cells, cultured cell lines, and sensitive cell models.
- Cell biology
- Primary cell research
- Immunology
- Cancer research
- Stem cell and regenerative medicine research
- Drug discovery and screening
- Cell culture workflow development
- Translational biomedical research
- Biobanking and sample management
Key Applications
The product supports routine and specialized cell cryopreservation workflows.
- Cryopreservation of animal primary cells
- Cryopreservation of established animal cell lines
- Serum-free cell banking
- Short-term or long-term cell storage workflows
- Cell recovery before culture expansion
- Cell storage for downstream functional assays
- Cryopreservation of cells sensitive to animal-origin components or DMSO
Specifications
| Specification | Details |
|---|---|
| Product Name | Serum-Free One-Step Cell Freezing Medium |
| Catalog No. | FG-BA3356 |
| Pack Size | 100 mL |
| Formulation Features | Serum-free, animal-origin-free, antibiotic-free, low DMSO |
| Compatible Samples | Animal primary cells and cell lines |
| Main Function | Cell cryopreservation |
| Workflow | Cell counting, centrifugation, resuspension in freezing medium, cryovial aliquoting, -80°C freezing, optional liquid nitrogen storage |
| Programmed Cooling Required | No |
| General Cryopreservation Density | 1 × 10⁶ cells/mL |
| Storage After Freezing Cells | Directly place cryovials at -80°C; transfer to liquid nitrogen after 24 hours for longer-term storage |
| Thawing Temperature | 37°C water bath |
| Example Thawing Centrifugation | 300 ×g for 5 minutes at 4°C |
| Required Instruments for Freezing | Horizontal centrifuge, cell counter, -80°C freezer |
| Required Instruments for Thawing | Horizontal centrifuge, water bath |
| Required Reagents for Thawing | RPMI 1640 medium or suitable culture medium |
| Required Consumables | Cell cryovials, low-adsorption pipette tips, centrifuge tubes, alcohol cotton balls |
| Product Storage | -20°C, protected from light |
| Shelf Life | One year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| Serum-Free One-Step Cell Freezing Medium | BA3356 | 100 mL |