In high-throughput single-cell sequencing, single-nucleus suspensions have emerged as a powerful alternative for overcoming the limitations of traditional single-cell preparation—particularly when working with complex tissues, large or irregularly shaped cells, lipid-rich or enzyme-dense samples, and structurally fragile specimens. Leveraging extensive expertise from dissecting over 10,000 samples across more than 150 tissue types, FireGene has independently developed an advanced nuclear suspension preparation kit. This innovative solution enables the efficient generation of high-quality single-nucleus suspensions, effectively bypassing the technical challenges associated with conventional tissue dissociation methods. As a result, it provides researchers with a robust and reliable approach for unlocking high-resolution insights from even the most challenging biological samples in single-cell sequencing workflows.
Product Advantages
Broad Application Scope
Designed for efficient isolation and extraction of cell nuclei from a wide range of sample types, including cell suspensions, fresh tissues, and frozen tissues derived from humans and other mammals. This versatility makes it ideal for diverse experimental needs.
Superior Performance
Enables the preparation of high-quality single-nucleus suspensions with excellent yield. The extracted nuclei maintain intact nuclear membranes and structural integrity, fully meeting the stringent requirements of downstream single-cell sequencing workflows.
Reliable and High-Quality Data Output
Delivers consistent and reproducible results. Validation studies using mouse brain and myocardial tissues have demonstrated outstanding quality control metrics, ensuring confidence in experimental outcomes.
Versatile Application Scenarios
Compatible with multiple advanced research applications, including single-nucleus RNA sequencing (snRNA-seq), single-cell ATAC sequencing (scATAC-seq), and other epigenomic and transcriptomic analyses.
Test Results
Experiments performed on cryopreserved mouse brain and myocardial tissues demonstrated that the FireGene Nuclear Suspension Preparation Kit efficiently produces high-quality nuclear suspensions. The resulting nuclei maintain integrity and yield sufficient material, fully meeting the stringent requirements for single-nucleus transcriptome sequencing (snRNA-seq) experiments. These results confirm the kit’s reliability and suitability for advanced single-cell sequencing applications.
Test Process:
Test results:
Quality Inspection Results of Single-Nucleus Suspensions Prepared from Different Tissues Using the Multi Tissue Nuclei Isolation Kit
Micrographs of Single-Nucleus Suspensions from Mouse Brain (Left) and Myocardium (Right)
Project Actual Measurement
At present, we have completed a large number of single-nucleus sequencing projects of frozen samples processed with this kit, involving many tissue types. The following shows part of the project data. The results show that the nucleus suspension processed by this kit has a high nucleated rate, low clumping rate, clean background, and intact nuclear membrane. After sequencing, the obtained data have a high median number of genes and a low mitochondrial ratio, showing excellent overall performance!
Data Performance of Nucleus Suspensions Prepared from Different Tissue Types in Some Actual Projects
Microscopic Inspection Results of Nucleus Suspensions Prepared from Different Tissue Types in Some Actual Projects
Single-Nucleus Sequencing Clustering Performance of Nucleus Suspensions Prepared from Different Tissue Types in Some Actual Projects
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