FireGene Plasma cfDNA Extraction Kit

FireGeneSKU: FG-0336

- Compatible with a wide range of plasma samples

- cfDNA purification for PCR, chip detection, and high-throughput sequencing

- No hazardous chemicals

- Quick and easy to use

- Simple workflow compatible with most instruments

Ship with ice packs

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Product Overview

Description

The kit employed the FireGene-developed magnetic bead purification technology and unique buffer system, which is suitable to extract cell-free DNA (cfDNA) from large-volume (1 mL~4mL) EDTA plasma, cell-free DNA sample preservation tube plasma, such as Streck Cell-Free DNA BCT non-invasive blood collection tube, CWBIO's cell-free DNA sample storage tubes, etc. The extraction process does not involve toxic reagents such as chloroform and phenol. The extracted cfDNA is stable and reliable quality with high purity. The extracted cfDNA can be used for conventional PCR, quantitative PCR, chip detection, downstream experiments such as high-throughput sequencing. The kit can also be efficiently used with the pipetting workstation and magnetic bar method magnetic bead automatic extraction system.


    Specifications

    Product Type Nucleic Acid Extraction/Purification
    Applications Conventional PCR, quantitative PCR, chip detection, downstream experiments such as high-throughput sequencing
    Compatible Sample Types EDTA plasma, cell-free DNA sample preservation tube plasma, etc.
    Target cfDNA
    Method Magnetic beads
    Prep Time
    Amount of Starting Material
    Supported Instruments Compatible with most automated nucleic acid extraction instruments, high-throughput compatible
    Number of Reactions 96
    Storage

    -20°C for Proteinase K

    2~8°C for Magnetic Bead Suspensions

    Room temperature for all other components

    Shelf-life 12 months

    Components

    - Lysis Buffer (LB) 400 mL
    - Wash Buffer (WB) 400mL
    - Elution Buffer (EB) 10 mL
    - Proteinase K 10 mL
    - Magnetic Bead Suspensions 8 mL
    - 20% SDS 20 mL

    Online Inquiry

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