FireGene Plasma cfDNA Extraction Kit

Description

The kit employed the FireGene-developed magnetic bead purification technology and unique buffer system, which is suitable to extract cell-free DNA (cfDNA) from large-volume (1 mL~4mL) EDTA plasma, cell-free DNA sample preservation tube plasma, such as Streck Cell-Free DNA BCT non-invasive blood collection tube, CWBIO's cell-free DNA sample storage tubes, etc. The extraction process does not involve toxic reagents such as chloroform and phenol. The extracted cfDNA is stable and reliable quality with high purity. The extracted cfDNA can be used for conventional PCR, quantitative PCR, chip detection, downstream experiments such as high-throughput sequencing. The kit can also be efficiently used with the pipetting workstation and magnetic bar method magnetic bead automatic extraction system.

Specifications

Product Type	Nucleic Acid Extraction/Purification
Applications	Conventional PCR, quantitative PCR, chip detection, downstream experiments such as high-throughput sequencing
Compatible Sample Types	EDTA plasma, cell-free DNA sample preservation tube plasma, etc.
Target	cfDNA
Method	Magnetic beads
Prep Time
Amount of Starting Material
Supported Instruments	Compatible with most automated nucleic acid extraction instruments, high-throughput compatible
Number of Reactions	48 rxns/96rxns
Storage	-Proteinase K and magnetic bead suspension should be stored at C2-8℃; other components should be stored at room temperature
Shelf-life	12 months

Kit Components

Components Name	10 times	Storage temperature
Lysis buffer 1	4.5mL	RT.
Lysis buffer 2	33.6mL	RT.
Proteinase K	1.7mL	2-8℃
Magnetic bead suspension C	0.9mL	2-8℃
Washing solution C1	11mL	RT.
Washing liquid C2	5mL	RT.
Elution Buffer C	800µL	RT.