Description
Product Introduction
This product is a recombinant mouse monoclonal antibody against β-actin, serving as a powerful tool for studying the intracellular distribution and cytoskeletal role of this protein. β-actin is a major protein in striated muscle fibers and a primary component of muscle thin filaments and cytoskeletal microfilaments. It is widely distributed, highly conserved, and has a molecular weight of 42–43 kDa; due to its relatively stable expression within cells, it is frequently used as an internal control for normalization.
Clone No. 23L215B7
Buffer 10 mM PBS (pH 7.4), 50% glycerol
Preservative 0.01% sodium azide
Applicable species
Human, rat, mouse, monkey, dog, chicken, hamster, rabbit, pig, sheep
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA
Recommended Concentration (The optimal concentration needs to be adjusted based on specific conditions.)
Western Blot: 1:5,000–1:10,000
IHC: 1:500
IF: 1:500–1:1,000
ELISA: 1:5,000–1:10,000
Precautions
1. To achieve optimal experimental results, please determine the best working dilution based on your specific experiment;
2. For your safety and health, please wear a lab coat and disposable gloves while handling the product;
3. This product is intended for research use only.