Description
FireGene Annelid Dissociation Kit is designed for preparation of single-cell suspensions from various annelid tissues, including earthworms, leeches, and sandworms. The kit uses mild enzymatic digestion to release cells from field-collected or laboratory-bred annelid samples. Prepared cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays. The protocol includes tissue washing, mincing, enzymatic digestion, filtration, washing, resuspension, and quality control.
Background Information
Annelids are used in developmental biology, regeneration, environmental biology, and comparative cell research. Tissue dissociation workflows help prepare cell suspensions from diverse annelid samples for downstream cellular analysis.
FireGene Annelid Dissociation Kit for Single-Cell Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Annelid biology
- Comparative cell biology
- Regeneration research
- Developmental biology
- Environmental and toxicology studies
- Invertebrate model research
- Single-cell biology
- Cell culture assay development
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Annelid tissue single-cell suspension preparation
- Single-cell sequencing
- Cell culture
- Cell viability and counting
- Cell-based assays
- Tissue heterogeneity studies
- Comparative organism research
Specifications
| Product Name | Annelid Dissociation Kit |
| Brand | FireGene |
| Catalog No. | FG-BA3335 |
| Kit Size | 10 reactions |
| Sample Type | Annelid tissues |
| Compatible Samples | Fresh tissues from annelids, including earthworms, leeches, and sandworms |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Preparation of annelid single-cell suspensions |
| Workflow | Enzymatic digestion, filtration, centrifugation, washing, resuspension, quality control |
| Downstream Applications | Single-cell sequencing, cell culture, cell-related assays |
| Digestion Conditions | 37°C for 0.5–2 hours |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | PBS, FBS, RPMI 1640 medium |
| Required Consumables | Low-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | -20°C |
| Shelf Life | Two years |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| ADS (Annelid Dissociation Solution) | FG-BA3335-A | 2 × 1.25 mL |