Description
FireGene Anti-Clumping Agent is designed to reduce cell aggregates in single-cell suspension samples derived from dissociated animal tissues. The treatment helps maintain a low aggregation rate, supporting smoother downstream workflows such as flow cytometry analysis, single-cell sequencing, cell culture, and other cell-related assays. The protocol uses a short 30°C incubation followed by washing and resuspension, with cell concentration adjusted according to downstream requirements.
Background Information
Cell clumping can reduce sample quality in single-cell workflows by affecting counting, sorting, sequencing loading, and downstream assay consistency. An anti-clumping treatment helps improve suspension uniformity before analysis or culture.
FireGene Anti-Clumping Agent for Single-Cell Suspensions provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Single-cell sample preparation
- Flow cytometry assay development
- Cell sorting workflows
- Primary tissue dissociation workflows
- Cell culture research
- Cancer and immunology research
- Drug discovery assays
- Translational biomedical research
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Reduction of cell aggregates
- Single-cell suspension cleanup
- Flow cytometry analysis
- Single-cell sequencing preparation
- Cell culture workflows
- Cell counting and quality control
- Post-dissociation sample processing
Specifications
| Product Name | Anti Clumping Agent |
| Brand | FireGene |
| Catalog No. | FG-BA3345 |
| Pack Size | 100 mL |
| Sample Type | Single-cell suspensions from dissociated animal tissues |
| Main Function | Reduction of cell aggregates |
| Workflow | Centrifugation, anti-clumping treatment, incubation, washing, resuspension, quality control |
| Downstream Applications | Flow cytometry analysis, single-cell sequencing, cell culture, cell-related assays |
| Treatment Conditions | 30°C for 20 minutes |
| Recommended Cell Concentration During Treatment | Not more than 2,000 cells/μL |
| Required Instruments | Water bath, benchtop centrifuge, cell counter |
| Required Reagents | PBS, FBS |
| Required Consumables | Low-retention pipette tips, centrifuge tubes |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | 4°C, protected from light |
| Shelf Life | One year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| Anti Clumping Agent | FG-BA3345 | 100 mL |