Description
FireGene Blood Vascular Dissociation Kit Plus is an optimized enzyme-based kit for dissociation of blood vascular tissues from humans and other large mammals. The workflow is designed to improve dissociation efficiency while preserving cell viability, helping address the challenges of vascular tissue processing. Prepared cells can be used for single-cell sequencing, cell sorting, cell culture, and other cellular experiments. The protocol uses a two-step digestion workflow with quality checks during processing.
Background Information
Vascular tissues can be difficult to dissociate because of extracellular matrix structure and tissue-specific cellular organization. A vascular-focused dissociation workflow helps researchers prepare single-cell suspensions for studying endothelial cells, smooth muscle cells, immune cells, and other vascular cell populations.
FireGene Blood Vascular Dissociation Kit Plus for Single-Cell Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Vascular biology
- Cardiovascular disease research
- Endothelial cell biology
- Smooth muscle cell research
- Atherosclerosis and vascular inflammation
- Angiogenesis research
- Tissue remodeling studies
- Single-cell biology
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Vascular tissue single-cell suspension preparation
- Single-cell sequencing
- Flow cytometry and cell sorting
- Primary vascular cell culture
- Cell viability and cell counting
- Vascular tissue heterogeneity analysis
- Cardiovascular disease model research
Specifications
| Product Name | Blood Vascular Dissociation Kit (Plus) |
| Brand | FireGene |
| Catalog No. | FG-BA3337 |
| Kit Size | 10 reactions |
| Sample Type | Blood vascular tissue |
| Compatible Samples | Fresh vascular tissues from humans and other large mammals |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Preparation of vascular tissue single-cell suspensions |
| Workflow | Two-step enzymatic digestion, dilution, filtration, centrifugation, washing, resuspension |
| Downstream Applications | Single-cell sequencing, cell sorting, cell culture |
| Digestion Conditions | 37°C; 20–30 minutes with BVDSP 1, then 30 minutes to 2 hours with BVDSP 2 |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | PBS, FBS, RPMI 1640 medium |
| Required Consumables | Low-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | -20°C |
| Shelf Life | Two years |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| BVDSP 1 (Blood Vascular Dissociation Solution (Plus) 1) | FG-BA3337-A | 30 mL |
| BVDSP 2 (Blood Vascular Dissociation Solution (Plus) 2) | FG-BA3337-A | 30 mL |