Description
FireGene Brain Tissue Cell Debris Removal Kit is designed to remove debris from single-cell suspensions generated after mammalian brain tissue dissociation. The workflow helps reduce debris background before downstream experiments such as flow cytometry analysis and single-cell sequencing. The protocol uses Brain Tissue Cell Debris Removal Solution with layered centrifugation, followed by debris layer removal, washing, optional red blood cell lysis, optional 20 μm filtration when clumping is present, and final quality control.
Background Information
Brain tissue dissociation often produces debris that can affect downstream cell analysis. A dedicated debris removal step helps improve sample background before flow cytometry, single-cell sequencing, and other cell-based workflows.
FireGene Brain Tissue Cell Debris Removal Kit for Single-Cell Cleanup provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Neuroscience research
- Brain tissue dissociation workflows
- Neuroinflammation studies
- Neurodegeneration research
- Glial and neuronal cell analysis
- Single-cell biology
- Flow cytometry assay development
- Translational neurobiology
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Brain tissue cell debris removal
- Single-cell suspension cleanup
- Flow cytometry analysis
- Single-cell sequencing preparation
- Cell counting and quality control
- Post-dissociation sample processing
- Neural cell population analysis
Specifications
| Product Name | Brain Tissue Cell Debris Removal Kit |
| Brand | FireGene |
| Catalog No. | FG-BA3330 |
| Kit Size | 10 reactions |
| Sample Type | Single-cell suspensions from mammalian brain tissue |
| Main Function | Removal of debris from brain tissue cell suspensions |
| Workflow | Centrifugation, DRS treatment, layered separation, debris removal, washing, optional RBC lysis, optional filtration, resuspension |
| Downstream Applications | Flow cytometry analysis, single-cell sequencing |
| Separation Conditions | 4°C, 3000 ×g, 20 minutes |
| Required Instruments | Horizontal centrifuge |
| Required Reagents | PBS, FBS |
| Required Consumables | Low-adhesion pipette tips, centrifuge tubes, 20 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | 4°C, protected from light |
| Shelf Life | One year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| DRS (Brain Tissue Cell Debris Removal Solution) | FG-BA3330 | 10 mL |