FireGene Cochlea Dissociation Kit – Inner Ear Cell Prep

Overview

The FireGene Cochlea Dissociation Kit is a specialized enzymatic solution optimized to isolate single cells from delicate cochlear tissue. Designed for use in single-cell sequencing and auditory research, this kit ensures high-viability cell suspensions for advanced studies in hearing loss, balance disorders, and neurobiology.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Understanding inner ear biology and hearing mechanisms requires accurate dissociation of cochlear tissue into single cells.
  • Traditional methods often fail to isolate hair cells and auditory neurons without damage.
  • The kit supports:
    • Research on age-related hearing loss, Ménière’s disease, and genetic hearing impairments.
    • Identification of gene expression profiles linked to hair cell degeneration and regeneration.
    • Development of targeted therapies for restoring hearing and treating balance dysfunction.

• Background of Technological Development:

  • Mechanical or chemical dissociation methods often compromise cell integrity and yield.
  • This kit employs:
    • A precise enzymatic digestion strategy that matches the biochemical makeup of cochlear ECM.
    • Carefully tuned enzyme types, concentrations, and reaction times.
    • A robust protocol to achieve efficient, reproducible cell release with excellent viability.

Detection Principle

• Based on a synergistic enzymatic digestion process tailored to the cochlea:
  • Cochlear tissue is finely sectioned for maximal enzyme exposure.
  • Reagents are added in sequence under controlled temperature and timing.
  • The enzymes act to degrade ECM components and loosen intercellular adhesions.
• Final Output:
  • A clean, viable single-cell suspension ideal for scRNA-seq, auditory cell profiling, and regenerative studies.
    

Specifications

Kit Components