Description
FireGene Corneal Dissociation Kit is designed for the isolation and preparation of cell suspensions from corneal tissue. The kit uses enzyme-based digestion to gently and rapidly disrupt extracellular matrix and release cells. Prepared cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays. The protocol includes digestion, filtration through a 70 μm cell strainer, washing, resuspension, optional 20 μm filtration when clumps are present, and quality control.
Background Information
Corneal tissue contains specialized epithelial, stromal, endothelial, and immune-associated cells. Preparing a cell suspension helps researchers analyze corneal cell populations, tissue responses, and disease-related cellular changes.
FireGene Corneal Dissociation Kit for Single-Cell Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Ocular biology
- Corneal tissue research
- Eye disease models
- Epithelial and stromal cell biology
- Inflammation and wound healing studies
- Regenerative medicine
- Single-cell biology
- Cell culture assay development
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Corneal cell suspension preparation
- Single-cell sequencing
- Cell culture
- Cell viability and counting
- Cell-based assays
- Corneal tissue heterogeneity analysis
- Ocular disease mechanism research
Specifications
| Product Name | Corneal Dissociation Kit |
| Brand | FireGene |
| Catalog No. | FG-BA3357 |
| Kit Size | 10 reactions |
| Sample Type | Corneal tissue |
| Compatible Samples | Fresh corneal tissue |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Preparation of corneal cell suspensions |
| Workflow | Enzymatic digestion, filtration, centrifugation, washing, optional clump filtration, resuspension |
| Downstream Applications | Single-cell sequencing, cell culture, cell-related assays |
| Digestion Conditions | 37°C for 20–30 minutes |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | PBS, FBS, RPMI 1640 culture medium |
| Required Consumables | Low-adsorption pipette tips, centrifuge tubes, 20 μm and 70 μm cell strainers |
| Storage | Buffer A and Enzyme B: -20°C; Enzyme C: 4°C, protected from light |
| Shelf Life | Buffer A and Enzyme B: 2 years; Enzyme C: 1 year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| Buffer A | FG-BA3357-A | 21.6 mL |
| Enzyme B | FG-BA3357-B | 8 mL |
| Enzyme C | FG-BA3357-C | 100 μL |