Description
FireGene Fallopian Tube Dissociation Kit is designed for enzymatic dissociation of mammalian fallopian tube tissues, including human and mouse samples. The kit supports preparation of single-cell suspensions with simple operation and stable performance. Prepared cells can be used for single-cell sequencing, cell sorting, cell culture, and related cellular experiments. The protocol uses a two-step digestion workflow with FTDS 1 and FTDS 2, followed by filtration, washing, resuspension, and quality control.
Background Information
Fallopian tube tissue contains epithelial, stromal, immune, and vascular components that can be difficult to dissociate consistently. A tissue-specific two-step digestion workflow helps prepare cell suspensions for reproductive biology and disease research.
FireGene Fallopian Tube Dissociation Kit for Single-Cell Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Reproductive biology
- Fallopian tube tissue research
- Gynecological disease studies
- Epithelial cell biology
- Cancer origin and tumor microenvironment research
- Single-cell biology
- Cell culture assay development
- Translational biomedical research
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Fallopian tube single-cell suspension preparation
- Single-cell sequencing
- Flow cytometry and cell sorting
- Primary tissue cell culture
- Cell viability and counting
- Tissue heterogeneity analysis
- Disease mechanism research
Specifications
| Product Name | Fallopian Tube Dissociation Kit |
| Brand | FireGene |
| Catalog No. | FG-BA3336 |
| Kit Size | 10 reactions |
| Sample Type | Mammalian fallopian tube tissue |
| Compatible Samples | Human and mouse fallopian tube tissues |
| Recommended Starting Material | Approximately 200 mg fresh tissue |
| Main Function | Preparation of fallopian tube single-cell suspensions |
| Workflow | Two-step enzymatic digestion, filtration, centrifugation, washing, resuspension, quality control |
| Downstream Applications | Single-cell sequencing, cell sorting, cell culture |
| Digestion Conditions | 37°C; 5–10 minutes with FTDS 1, then 15–45 minutes with FTDS 2 |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | PBS, FBS, RPMI 1640 medium |
| Required Consumables | Low-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | -20°C |
| Shelf Life | Two years |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| FTDS 1 (Fallopian Tube Dissociation Solution 1) | FG-BA3336-A | 2 ×1.1 mL |
| FTDS 2 (Fallopian Tube Dissociation Solution 2) | FG-BA3336-B | 4 ×1.35 mL |