FireGene Cell RNA Extraction Kit

FireGeneSKU: FG-0410

High purity without DNA contamination

Repeatable results between samples with minimum variance

No hazardous chemicals

Quick and easy to use

Compatible with high-throughput automatic nucleic acid extraction instruments

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Product Overview

Description

This kit provides a simple and fast solution for the extraction of total RNA from cultured cells. The kit is based on superparamagnetic magnetic particle purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. The resulting RNA can be directly used in downstream experiments such as RT-PCR, fluorescence quantification, next-generation sequencing, viral RNA detection, and high-throughput sequencing. The kit can also be efficiently used with the liquid workstation and the magnetic bar method magnetic bead automatic extraction system for fast and large sample extraction.

Specifications

Product Type RNA Extraction
Applications RT-PCR, fluorescence quantification, next-generation sequencing, viral RNA detection, and high-throughput sequencing
Compatible Sample Types Cells
Target Total RNA
Method Scalable, flexible format with magnetic beads
Prep Time < 60 minutes
Amount of Starting Material 5x104 to 5x106 cells
Supported Instruments Compatible with most automated nucleic acid extraction instruments and pipetting workstations, high-throughput compatible
Number of Reactions 96
Storage

-20°C for DNase â…  and DNase dilution solution

2~8°C for Magnetic bead suspensions

Room temperature for all other components

Shelf-life 12 months

Components

- Magnetic Bead Suspensions 2 mL
- Lysis Buffer (LB) 40 mL
- Wash Buffer 1 (WB1) 60 mL
- Wash Buffer 2 (WB2) 13 mL
- Eluant (RFW) 5 mL
- DNase Ⅰ 1 mL
- DNase Dilution Solution 1.5 mL

Files Available (Login to View/Download)

- CE Marking Certification Letter
- EU Declaration of Conformity
- User Manual

Online Inquiry

Inquire the FireGene Cell RNA Extraction Kit by filling out the form below. Free shipping within the United States for lyophilized products.

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