Description
Product Introduction
This product is a recombinant rabbit monoclonal antibody. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is a key enzyme involved in glycolysis, composed of four 36 kDa subunits. Beyond its role in glycolysis, evidence indicates that GAPDH in mammalian cells participates in various intracellular biochemical processes, including membrane fusion, microtubule bundling, phosphotransferase activation, nuclear RNA export, DNA replication, and DNA repair. GAPDH is highly expressed in almost all tissues and is commonly used as an internal loading control in Western blot assays.
Clone No. 29Q12D64
Buffer 10 mM PBS (pH 7.4), 50% glycerol
Preservative 0.02% sodium azide
Applicable species
Human, rat, mouse, monkey, dog, chicken, hamster, rabbit, pig, sheep, insect, yeast
Applications
Western Blot(WB)、Immunohistochemistry(IHC)、Immunocytochemistry (ICC)/
Immunofluorescence(IF)、Immunoprecipitation(IP)、Flow Cytometry(FC)
Recommended Concentration (The optimal concentration needs to be adjusted based on specific conditions.)
WB：1:5,000~1:50,000; IHC：1:100~1:500; ICC/IF 1:100~1:250; IP 1:50; FC 1:50。