FireGene Gastrointestinal Dissociation Kit – Gut Cell Isolation

Overview

The FireGene Gastrointestinal Dissociation Kit is a high-performance enzymatic solution designed to isolate viable single-cell suspensions from GI tissues. Optimized for applications like single-cell sequencing, immune profiling, and gut microbiota research, this kit delivers exceptional results for complex and fragile gastrointestinal samples.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Single-cell sequencing of GI tissue is critical for understanding diseases such as IBD (inflammatory bowel disease), colorectal cancer, and intestinal fibrosis.
  • Conventional methods do not fully preserve the diversity of epithelial, stromal, and immune cells.
  • This kit enables:
    • Detailed characterization of cell subsets involved in inflammation, cancer, and tissue repair.
    • Identification of biomarkers and therapeutic targets for precision medicine.
    • Support for regenerative and microbiome-associated GI research.

• Background of Technological Development:

  • Traditional techniques often result in low cell viability and inefficient dissociation.
  • FireGene resolves these limitations by:
    • Using a targeted enzymatic system tailored for gut ECM and cell junctions.
    • Optimizing reaction conditions for reproducibility across different GI tissue types.
    • Delivering high cell recovery rates suitable for sensitive downstream applications.

Detection Principle

• Uses a multi-step enzymatic digestion approach:
  • GI tissue is cut into small fragments for uniform reagent exposure.
  • Enzymes are applied sequentially, breaking down extracellular matrix and cellular adhesions.
  • The process occurs under carefully regulated incubation conditions.
• Outcome:
  • A high-quality, viable single-cell suspension ideal for scRNA-seq, flow cytometry, and functional GI assays.

Specifications

Kit Components