FireGene Heart Dissociation Kit – Cardiomyocyte-Ready

Overview

The FireGene Heart Dissociation Kit is a specialized enzymatic solution optimized for the dissociation of heart tissue into high-viability single-cell suspensions. This kit is ideal for cardiology research, enabling precise cellular analysis in conditions such as cardiomyopathy, heart failure, and cardiac regeneration.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Single-cell sequencing of heart tissue is essential for understanding the cellular complexity of cardiovascular diseases.
  • Traditional methods struggle to isolate diverse cardiac cells including cardiomyocytes, fibroblasts, endothelial cells, and immune populations.
  • This kit supports:
    • Analysis of cellular heterogeneity and intercellular signaling.
    • Discovery of biomarkers and drug targets related to heart failure and inflammation.
    • Research into heart regeneration, development, and personalized therapy.

• Background of Technological Development:

  • Conventional dissociation methods often result in low cell viability and incomplete tissue breakdown.
  • FireGene improves outcomes through:
    • Advanced enzymatic blends tailored for dense cardiac ECM.
    • Carefully optimized concentration, temperature, and reaction time protocols.
    • A workflow that ensures reproducible, high-yield single-cell preparation for downstream applications.

Detection Principle

• Implements a stepwise enzymatic digestion process:
  • Heart tissue is minced into small fragments.
  • Enzyme reagents are added in sequence under precisely managed incubation conditions.
  • The digestion gently breaks down the extracellular matrix and cell junctions, releasing intact cells.
• Result:
  • A clean, viable single-cell suspension suitable for scRNA-seq, cardiac lineage tracing, functional assays, and drug screening.

Specifications

Kit Components