Description
FireGene Human PBMC Isolation Solution is designed for the isolation of lymphocytes from human peripheral blood. The workflow prepares high-viability, low-impurity lymphocyte single-cell suspensions for flow cytometry analysis, single-cell sequencing, cell culture, and other cell-related assays. The protocol uses density-based separation with horizontal centrifugation, followed by mononuclear cell collection, optional red blood cell lysis, washing, and resuspension for downstream use.
Background Information
Human PBMC isolation is widely used in immunology, translational research, and clinical sample processing workflows. Clean lymphocyte suspensions help improve downstream analysis where cell viability and sample background are important.
FireGene Human PBMC Isolation Solution for Lymphocyte Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Human immunology
- Peripheral blood immune profiling
- Inflammation and infection research
- Cancer immunology
- Translational biomedical research
- Biomarker discovery
- Single-cell biology
- Flow cytometry assay development
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Human PBMC isolation
- Lymphocyte single-cell suspension preparation
- Flow cytometry analysis
- Single-cell sequencing
- Cell culture and cell-based assays
- Immune cell profiling
- Peripheral blood sample processing
Specifications
| Product Name | PBMC Isolation Solution, human |
| Brand | FireGene |
| Catalog No. | FG-BA3332 |
| Pack Size | 100 mL |
| Sample Type | Human peripheral blood |
| Compatible Samples | Diluted human blood samples |
| Main Function | Isolation of lymphocytes / PBMCs |
| Workflow | Sample dilution, density-layer separation, centrifugation, mononuclear cell collection, optional RBC lysis, washing, resuspension |
| Downstream Applications | Flow cytometry analysis, single-cell sequencing, cell culture, cell-related assays |
| Centrifugation for Separation | 20°C, 700 ×g, 30 minutes |
| Required Instruments | Horizontal centrifuge, cell counter |
| Required Reagents | PBS, FBS |
| Required Consumables | Low-adsorption pipette tips, 15 mL centrifuge tubes |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | 4°C, protected from light |
| Shelf Life | One year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| Human PBMC Isolation Solution | FG-BA3332 | 100 mL |