FireGene Kidney Dissociation Kit – Enzymatic Single-Cell Isolation

Overview

The FireGene Kidney Dissociation Kit is a precision-engineered enzymatic system for the efficient isolation of single cells from kidney tissue. Designed to support cutting-edge research into renal function and pathology, this kit delivers high-viability suspensions for single-cell sequencing, flow cytometry, and biomarker discovery.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Kidney diseases such as acute kidney injury (AKI) and chronic kidney disease (CKD) require cellular-level investigation to understand their progression.
  • Traditional dissociation approaches do not capture the full range of renal cell types and often lead to low yield.
  • This kit enables:
    • High-resolution profiling of renal tubule cells, podocytes, immune cells, and more.
    • Discovery of biomarkers related to renal fibrosis, inflammation, and tissue remodeling.
    • Accelerated development of targeted treatments for nephrological disorders.

• Background of Technological Development:

  • Traditional dissociation techniques are limited by low efficiency and cell damage.
  • FireGene addresses these challenges by:
    • Using refined enzymatic formulations matched to the unique extracellular matrix of kidney tissue.
    • Tuning enzyme concentration, incubation time, and buffer composition for optimal results.
    • Ensuring maximum recovery of viable, functional cells ready for downstream workflows.

Detection Principle

• Utilizes a stepwise enzymatic digestion process:
  • Kidney tissue is finely chopped and exposed to carefully formulated enzymatic reagents.
  • Reagents act in synergy under controlled temperature and timing conditions.
  • The enzymes degrade the extracellular matrix and intercellular connections, enabling complete tissue dissociation.
• Outcome:
  • A high-purity, viable single-cell suspension suitable for scRNA-seq, functional assays, and renal disease modeling.

Specifications

Kit Components