FireGene Lung Dissociation Kit – scRNA-seq Compatible

Overview

The FireGene Lung Dissociation Kit is specially formulated to isolate high-viability single cells from lung tissue using a powerful enzymatic protocol. Ideal for research in pulmonary diseases and immune profiling, this kit provides clean, consistent cell suspensions for advanced applications such as scRNA-seq and cytometry.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Research into lung diseases like COPD, pulmonary fibrosis, and lung cancer demands single-cell resolution.
  • Traditional dissociation methods fail to preserve fragile lung cell types and introduce cellular stress.
  • This kit enables:
    • Identification of alveolar, endothelial, epithelial, and immune cells within lung tissue.
    • Discovery of new biomarkers and drug targets for precision medicine.
    • Deep analysis of cellular responses to disease and therapy.

• Background of Technological Development:

  • Addresses the inefficiencies and limitations of conventional dissociation.
  • Employs:
    • Optimized enzyme combinations specific to lung matrix composition.
    • Controlled protocols for timing, temperature, and concentration.
    • Enhanced cell recovery, reduced cellular stress, and high reproducibility.

Detection Principle

• Utilizes a synergistic enzymatic digestion process:
  • Lung tissue is sectioned into small fragments.
  • A stepwise reagent application is performed under precisely managed incubation conditions.
  • Enzymes break down the extracellular matrix and liberate single cells.
• Result:
  • A high-quality, viable single-cell suspension ready for sequencing, cytometry, or cell culture workflows.

Specifications

Kit Components