|Magic High-Fidelity DNA Polymerase is a novel B-family DNA polymerase, engineered to have increased affinity for DNA. The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared with wild-type B-family DNA polymerases. In addition, the ability to amplify long targets, as well as GC- and AT-rich targets, is significantly improved. Magic High-Fidelity DNA Polymerase has 5â€™→3â€™ polymerase and 3â€™→5â€™ exonuclease (proofreading) activity, but no 5â€™→3â€™ exonuclease activity. The strong 3â€™→5â€™ exonuclease activity results in superior accuracy during DNA amplification, lending to Magic High-Fidelity DNA Polymerase the lowest published error rate of all B-family DNA polymerases (1 error per 3.6 x 106 nucleotides incorporated). This fidelity is approximately 100 times higher than that of wild-type Taq DNA polymerase, and up to 10 times higher than that of other B-family DNA polymerases and polymerase blends.
In Magic High-Fidelity 2X PCR Master Mix with HF Buffer, Magic High-Fidelity DNA Polymerase is supplied in a convenient 2X Master Mix format, containing all reaction components except primers and template. The Master Mix contains Magic High-Fidelity DNA Polymerase (1 U per 50 ÂµL reaction) in a proprietary reaction buffer containing dNTPs (0.3 mM of each dNTP at 1X), MgCl2 (1.5 mM at 1X) and stabilizers. Magic High-Fidelity 2X PCR Master Mix with HF Buffer is designed for routine, high-fidelity PCR of a wide range of targets and fragment sizes. In addition, Magic High-Fidelity DNA polymerase requires significantly shorter reaction times than wild-type B-family DNA polymerases.
DNA fragments generated with Magic High-Fidelity 2X PCR Master Mix may be used for routine downstream analysis and applications, including restriction enzyme digestion, cloning and sequencing. PCR products generated with Magic High-Fidelity 2X PCR Master Mix are blunt-ended, but may be 3â€™-dA-tailed for cloning into TA cloning vectors.