| Description |
Magic High-Fidelity DNA Polymerase is a novel B-family DNA polymerase, engineered to have increased affinity for DNA. The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared with wild-type B-family DNA polymerases. In addition, the ability to amplify long targets, as well as GC- and AT-rich targets, is significantly improved. In addition, Magic High-Fidelity DNA Polymerase requires significantly shorter reaction times than wild-type B-family DNA polymerases.
Magic High-Fidelity DNA Polymerase has 5’→3’ polymerase and 3’→5’ exonuclease (proofreading) activity, but no 5’→3’ exonuclease activity. The strong 3’→5’ exonuclease activity results in superior accuracy during DNA amplification, lending to Magic High-Fidelity DNA Polymerase the lowest published error rate of all B-family DNA polymerases (1 error per 3.6 x 106 nucleotides incorporated). This fidelity is approximately 100 times higher than that of wild-type Taq DNA polymerase, and up to 10 times higher than that of other B-family DNA polymerases and polymerase blends.
DNA fragments generated with Magic High-Fidelity DNA Polymerase may be used for routine downstream analysis and applications, including restriction enzyme digestion, cloning and sequencing. PCR products generated with Magic High-Fidelity DNA Polymerase are blunt-ended, but may be 3’-dA-tailed for cloning into TA cloning vectors. |
| Source |
An E.coli strain carries the Magic High-Fidelity DNA Polymerase gene. |
| Applications |
High Fidelity PCR, High throughput PCR , Routine PCR |
| Size |
100 U / 1000 U / 5000 U |
| Concentration |
2,000 U/ml |
| Components |
Magic High-Fidelity DNA Polymerase (2,000 U/ml); 5X Magic High-Fidelity DNA Polymerase HF Buffer; 5X Magic High-Fidelity DNA Polymerase GC Buffer; MgCl2 Solution |
| Unit Definition |
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C |
| Usage |
For Research Use Only |
| Storage |
Storage Conditions: 20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, 1X stabilizers, pH 7.4 @ 25°C. Store the Magic High-Fidelity DNA Polymerase at -20°C. Please avoid repeated freeze-thaw cycles. |
