| Description |
MagicTaq DNA Polymerase is an optimized blend of Taq klenow and Deep Ocean DNA Polymerases. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3'- 5 'exonuclease activity of Deep Ocean DNA Polymerase increases the fidelity and robust amplification of Taq klenow DNA Polymerase. The convenient FirstTaq 2X Master Mix with GC Buffer with Dye formulation contains dNTPs, MgSO4, buffer components and stabilizers, as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands. |
| Applications |
Multiplex PCR, PCR , Specialty PCR, Routine PCR |
| Size |
100 rxn / 500 rxn |
| Concentration |
2x |
| Components |
2X PCR Master Mix with GC Buffer with Dye; High GC Enhancer |
| Usage |
For Research Use Only |
| Storage |
Store the 2X PCR Master Mix with GC Buffer with Dye at -20°C. Please avoid repeated freeze-thaw cycles. |
