Description
FireGene Mouse PBMC Isolation Solution is designed for the isolation of lymphocytes from mouse peripheral blood. The workflow uses density-based layer separation to prepare high-viability, low-impurity lymphocyte single-cell suspensions for downstream analysis. Prepared cells can be used for flow cytometry analysis, single-cell sequencing, cell culture, and other cell-related assays. The protocol includes blood dilution, controlled centrifugation, mononuclear cell collection, optional red blood cell lysis, washing, and final cell resuspension.
Background Information
Peripheral blood mononuclear cell isolation is a key step for immune profiling and blood-based cellular analysis. For mouse studies, clean lymphocyte suspensions help support reliable downstream analysis in immunology, disease models, and single-cell workflows.
FireGene Mouse PBMC Isolation Solution for Lymphocyte Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Mouse immunology
- Peripheral blood cell analysis
- Inflammation research
- Infectious disease models
- Cancer immunology
- Preclinical disease models
- Single-cell biology
- Flow cytometry assay development
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Mouse PBMC isolation
- Lymphocyte single-cell suspension preparation
- Flow cytometry analysis
- Single-cell sequencing
- Cell culture and cell-based assays
- Immune cell profiling
- Peripheral blood sample processing
Specifications
| Product Name | PBMC Isolation Solution, mouse |
| Brand | FireGene |
| Catalog No. | FG-BA3331 |
| Pack Size | 100 mL |
| Sample Type | Mouse peripheral blood |
| Compatible Samples | Diluted mouse blood samples |
| Main Function | Isolation of lymphocytes / PBMCs |
| Workflow | Sample dilution, density-layer separation, centrifugation, mononuclear cell collection, optional RBC lysis, washing, resuspension |
| Downstream Applications | Flow cytometry analysis, single-cell sequencing, cell culture, cell-related assays |
| Centrifugation for Separation | 20°C, 700 ×g, 30 minutes |
| Required Instruments | Horizontal centrifuge, cell counter |
| Required Reagents | PBS, FBS |
| Required Consumables | Low-adsorption pipette tips, 15 mL centrifuge tubes |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | 4°C, protected from light |
| Shelf Life | One year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| Mouse PBMC Isolation Solution | FG-BA3331 | 100 mL |