Description
FireGene Multi Tissue Dissociation Kit Plus is designed for the isolation and preparation of single-cell suspensions from highly metabolically active and pathological mammalian tissue samples. The kit uses an enzyme-based workflow to gently and rapidly degrade extracellular matrix and release cells. Prepared single-cell suspensions are suitable for single-cell sequencing, cell culture, and other cell-related assays. The protocol supports fresh tissue processing with adjustable digestion time based on tissue type, species, and sample condition.
Background Information
Highly active or pathological tissues can vary in matrix density, cellular composition, and tissue condition. A flexible dissociation workflow helps researchers adjust digestion time while preparing usable single-cell suspensions for downstream analysis.
FireGene Multi Tissue Dissociation Kit Plus for Single-Cell Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Mammalian tissue biology
- Disease model research
- Pathological tissue analysis
- Single-cell biology
- Cancer and inflammation research
- Drug discovery and screening
- Cell culture workflow development
- Translational biomedical research
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Single-cell RNA sequencing
- Flow cytometry and cell sorting
- Primary cell culture
- Cell viability and cell counting
- Cell-type composition analysis
- Cell-based functional assays
- Tissue heterogeneity studies
Specifications
| Product Name | Multi Tissue Dissociation Kit (Plus) |
| Brand | FireGene |
| Catalog No. | FG-BA3348 |
| Kit Size | 10 reactions |
| Sample Type | Highly metabolically active and pathological mammalian tissue samples |
| Compatible Samples | Fresh tissue |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Preparation of single-cell suspensions |
| Workflow | Enzymatic digestion, filtration, centrifugation, washing, resuspension, quality control |
| Downstream Applications | Single-cell sequencing, cell culture, cell-related assays |
| Digestion Conditions | 37°C for 10–20 minutes; 15–40 minutes for pathological tissues |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | PBS, FBS, RPMI 1640 medium |
| Required Consumables | Low-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | -20°C |
| Shelf Life | Two years |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| MTDSP (Multi Tissue Dissociation Solution (Plus)) | FG-BA3348 | 30 mL |