Description
FireGene Muscle Nuclei Isolation Kit is designed for the isolation and extraction of nuclei from human or mammalian muscle tissue, including fresh and frozen samples. The kit uses surfactants to disrupt cell membranes and release nuclei while maintaining nuclear membrane integrity. Through tissue grinding, filtration, centrifugation, and density-layer separation, the workflow produces clean single-nucleus suspensions suitable for single-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, and related nuclear analysis experiments.
Background Information
Muscle tissue is dense and fibrous, which can make whole-cell dissociation challenging. Nuclei isolation offers a practical sample-preparation approach for muscle research, especially when working with frozen tissue or samples that are difficult to dissociate into intact viable cells.
This kit supports nuclei extraction from both fresh and frozen muscle tissue, helping researchers prepare single-nucleus suspensions for sequencing and molecular analysis workflows.
Research Areas
The kit is suitable for research fields involving muscle structure, cellular diversity, gene regulation, and tissue heterogeneity.
- Skeletal muscle biology
- Muscle development and regeneration
- Neuromuscular disease research
- Aging-related muscle studies
- Metabolic disease models
- Exercise physiology research
- Regenerative medicine
- Translational biomedical research
- Single-cell and single-nucleus multiomics
Key Applications
The prepared nuclei suspensions can be used in downstream experiments that require clean nuclear material.
- Single-nucleus RNA sequencing, or snRNA-seq
- Single-nucleus ATAC sequencing, or snATAC-seq
- Nuclear transcriptome analysis
- Chromatin accessibility studies
- Cell-type composition analysis
- Gene expression profiling from fresh or frozen muscle tissue
- Tissue heterogeneity and disease mechanism studies
Specifications
| Specification | Details |
|---|---|
| Product Name | Muscle Nuclei Isolation Kit |
| Catalog No. | FG-BA3315 |
| Kit Size | 10 reactions |
| Sample Type | Human or mammalian muscle tissue |
| Compatible Samples | Fresh and frozen muscle samples |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Isolation and extraction of cell nuclei |
| Workflow | Tissue grinding, lysis, filtration, centrifugation, density-layer separation, nuclei resuspension |
| Downstream Applications | Single-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, related nuclear analysis experiments |
| Required Instruments | Horizontal centrifuge, tissue grinder, cell counter |
| Required Tissue Tools | Surgical scissors, scalpels, forceps |
| Required Consumables | Grinding tubes with zirconium beads, 5 mL and 15 mL centrifuge tubes, 40 μm cell strainers, optional 20 μm cell strainers |
| Additional Reagent | RNase inhibitor for nuclear RNA-related experiments |
| Storage | Main reagents: 4°C, protected from light |
| Additive Storage | Additive Solution ① and Additive Solution ②: -20°C, protected from light |
| Shelf Life | One year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| Lysis Buffer | FG-BA3315-A | 10 mL |
| Pre-Suspension Buffer | FG-BA3315-B | 15 mL |
| Post-Suspension Buffer | FG-BA3315-C | 50 mL |
| PB 1 | FG-BA3315-D | 3 mL |
| PB 2 | FG-BA3315-E | 6 mL |
| PB 3 | FG-BA3315-F | 6 mL |
| Additive Solution ① | FG-BA3315-G | 4 mL |
| Additive Solution ② | FG-BA3315-H | 150 μL |