Description
FireGene Ovarian Dissociation Kit is designed for enzymatic dissociation of ovarian tissues from large mammals. The kit addresses the challenge of difficult ovarian tissue dissociation and supports preparation of single-cell suspensions with high viability and low fragmentation rates. Prepared cells can be used for single-cell sequencing, cell sorting, cell culture, and related cellular experiments. The protocol uses a two-step digestion workflow with ODS 1 and ODS 2, followed by filtration, washing, and quality control.
Background Information
Ovarian tissue contains follicles, stromal cells, immune cells, vascular cells, and other specialized cell populations. Tissue-specific dissociation helps prepare single-cell suspensions for reproductive biology, ovarian disease models, and cellular analysis.
FireGene Ovarian Dissociation Kit for Single-Cell Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Ovarian biology
- Reproductive biology
- Female reproductive disease research
- Ovarian cancer and tumor microenvironment studies
- Fertility and follicle research
- Single-cell biology
- Cell culture assay development
- Translational biomedical research
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Large mammal ovarian single-cell suspension preparation
- Single-cell sequencing
- Flow cytometry and cell sorting
- Primary ovarian cell culture
- Cell viability and counting
- Tissue heterogeneity analysis
- Disease mechanism studies
Specifications
| Product Name | Ovarian Dissociation Kit |
| Brand | FireGene |
| Catalog No. | FG-BA3351 |
| Kit Size | 10 reactions |
| Sample Type | Ovarian tissue from large mammals |
| Compatible Samples | Fresh ovarian tissue |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Preparation of ovarian single-cell suspensions |
| Workflow | Two-step enzymatic digestion, filtration, centrifugation, washing, resuspension, quality control |
| Downstream Applications | Single-cell sequencing, cell sorting, cell culture |
| Digestion Conditions | 37°C; 15–20 minutes with ODS 1, then 30 minutes to 1 hour with ODS 2 |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | PBS, FBS, RPMI 1640 medium |
| Required Consumables | Low-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | -20°C |
| Shelf Life | Two years |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| ODS 1 (Ovarian Dissociation Solution 1) | FG-BA3351-A | 2 ×1.1 mL |
| ODS 2 (Ovarian Dissociation Solution 2) | FG-BA3351-B | 2 ×1.25 mL |