Description
FireGene Ovarian Dissociation Kit is designed for enzymatic dissociation of ovarian tissues from large mammals. The kit addresses the challenge of difficult ovarian tissue dissociation and supports preparation of single-cell suspensions with high viability and low fragmentation rates. Prepared cells can be used for single-cell sequencing, cell sorting, cell culture, and related cellular experiments. The protocol uses a two-step digestion workflow with ODS 1 and ODS 2, followed by filtration, washing, and quality control.
Background Information
Ovarian tissue contains follicles, stromal cells, immune cells, vascular cells, and other specialized cell populations. Tissue-specific dissociation helps prepare single-cell suspensions for reproductive biology, ovarian disease models, and cellular analysis.
FireGene Ovarian Dissociation Kit for Single-Cell Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Ovarian biology
- Reproductive biology
- Female reproductive disease research
- Ovarian cancer and tumor microenvironment studies
- Fertility and follicle research
- Single-cell biology
- Cell culture assay development
- Translational biomedical research
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Large mammal ovarian single-cell suspension preparation
- Single-cell sequencing
- Flow cytometry and cell sorting
- Primary ovarian cell culture
- Cell viability and counting
- Tissue heterogeneity analysis
- Disease mechanism studies
Specifications
| Product Name | Ovarian Dissociation Kit |
| Brand | FireGene |
| Catalog No. | FG-BA3351 |
| Kit Size | 10 reactions |
| Sample Type | Ovarian tissue from large mammals |
| Compatible Samples | Fresh ovarian tissue |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Preparation of ovarian single-cell suspensions |
| Workflow | Two-step enzymatic digestion, filtration, centrifugation, washing, resuspension, quality control |
| Downstream Applications | Single-cell sequencing, cell sorting, cell culture |
| Digestion Conditions | 37°C; 15–20 minutes with ODS 1, then 30 minutes to 1 hour with ODS 2 |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | PBS, FBS, RPMI 1640 medium |
| Required Consumables | Low-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | -20°C |
| Shelf Life | Two years |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| ODS 1 (Ovarian Dissociation Solution 1) | FG-BA3351-A | 2 ×1.1 mL |
| ODS 2 (Ovarian Dissociation Solution 2) | FG-BA3351-B | 2 ×1.25 mL |
Product FAQ
Q1: What tissue types and species is the Ovarian Dissociation Kit compatible with?
A: The kit is designed for enzymatic dissociation of fresh ovarian tissue from large mammals. We recommend approximately 200 mg of starting tissue material per reaction for optimal digestion efficiency.
Q2: How long does the dissociation protocol take?
A: The two-step enzymatic workflow takes approximately 45 minutes to 1 hour 20 minutes total: 15–20 minutes with ODS 1 followed by 30 minutes to 1 hour with ODS 2 at 37°C, plus filtration, washing, and resuspension steps.
Q3: What downstream applications is this kit suitable for?
A: Single-cell suspensions prepared with this kit support single-cell sequencing (scRNA-seq), flow cytometry/cell sorting, primary ovarian cell culture, and cell viability/counting workflows.
Q4: What equipment and consumables do I need beyond the kit?
A: You'll need a water bath or hybridization oven, a horizontal centrifuge, PBS, FBS, RPMI 1640 medium, low-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, and 70 μm cell strainers. These are not included in the kit.
Q5: Do I need to remove red blood cells from my sample?
A: If your ovarian tissue prep contains significant blood contamination, we recommend using our Red Blood Cell Lysis Solution (Catalog No. FG-BA3311) alongside this kit for cleaner single-cell suspensions.
Q6: What sizes are available, and what's included?
A: The kit is available in 10-reaction and 50-reaction sizes. Each 10-rxn kit includes ODS 1 (2 × 1.1 mL, Cat# FG-BA3351-A) and ODS 2 (2 × 1.25 mL, Cat# FG-BA3351-B).
Q7: How should the kit be stored, and what's the shelf life?
A: Store at -20°C. The kit has a shelf life of two years from the manufacture date when stored properly.
Q8: Is this kit validated for clinical/diagnostic use?
A: No — this product is for Research Use Only (RUO) and is not intended for diagnostic or therapeutic use.
Q9: Can digestion time be adjusted for different tissue conditions?
A: Yes, digestion time can be modified based on your specific sample characteristics (e.g., tissue firmness, fibrosis) and should be optimized based on cell viability and single-cell suspension quality checks.
Q10: How do I get a custom quote or bulk pricing?
A: Fill out the online inquiry form on this page with your organization details and reaction volume needs. We also offer free sample requests and free shipping within the United States for lyophilized products.