FireGene Pancreas Dissociation Kit – High-Fidelity Enzymatic Digestion

Overview

The FireGene Pancreas Dissociation Kit is designed for the efficient enzymatic dissociation of pancreatic tissue into viable single-cell suspensions. Ideal for single-cell sequencing and disease modeling, this kit supports investigations into diabetes, pancreatitis, and pancreatic cancer with high precision and reproducibility.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Understanding pancreatic disease mechanisms, such as diabetes, chronic inflammation, and pancreatic cancer, requires detailed single-cell analysis.
  • Traditional methods do not fully preserve islet cells, acinar cells, and other fragile populations.
  • This kit enables:
    • Isolation and profiling of pancreatic cell types for biomarker discovery.
    • Improved insight into immune response, fibrosis, and beta-cell function.
    • Development of targeted therapies and regenerative strategies for pancreatic diseases.

• Background of Technological Development:

  • FireGene overcomes the limitations of low cell yield and viability by using:
    • A proprietary enzymatic formulation designed specifically for pancreatic ECM.
    • Optimized conditions for incubation time, temperature, and enzyme concentration.
    • A workflow that preserves cell integrity, ensuring consistent and high-quality single-cell suspensions.

Detection Principle

• Based on a synergistic enzymatic digestion process:
  • Pancreatic tissue is minced and treated sequentially with prepared reagents.
  • The digestion proceeds under controlled conditions, breaking down ECM components and intercellular junctions.
• Final Result:
  • A clean, high-viability single-cell suspension ideal for use in scRNA-seq, proteomics, and pancreatic disease research.

Specifications

Kit Components