Description
FireGene Serum-Free and DMSO-Free Cell Freezing Medium is designed for cryopreservation of animal primary cells and immortalized cell lines that are sensitive to animal-origin components or DMSO. The formulation is serum-free, animal-origin-free, DMSO-free, and non-toxic. Cells are resuspended in the freezing medium, aliquoted into cryovials, placed in a programmable freezing container for controlled-rate cooling at approximately 1°C/min, and stored at -80°C before optional transfer to liquid nitrogen for longer-term storage.
Background Information
DMSO is commonly used in cell freezing media, but some primary cells and sensitive cell lines may require alternatives that avoid DMSO and animal-origin components. A serum-free and DMSO-free freezing medium can help support cell banking workflows where a clearer culture background or reduced exposure to undefined components is preferred.
FireGene Serum-Free and DMSO-Free Cell Freezing Medium provides a defined cryopreservation option for animal primary cells and immortalized cell lines that are sensitive to DMSO or animal-origin materials.
Research Areas
This freezing medium is suitable for cell culture and cryopreservation workflows across multiple research fields.
- Cell biology
- Primary cell research
- Immunology
- Cancer research
- Stem cell and regenerative medicine research
- Drug discovery and screening
- Translational biomedical research
- Cell banking and biopreservation
- Assay development using sensitive cell models
Key Applications
The product supports routine and specialized cryopreservation workflows where serum-free and DMSO-free conditions are needed.
- Cryopreservation of animal primary cells
- Cryopreservation of immortalized animal cell lines
- DMSO-free cell freezing
- Serum-free cell banking
- Preservation of cells sensitive to animal-origin components
- Cell recovery before culture expansion
- Long-term storage after transfer to liquid nitrogen
- Cell storage for downstream functional assays
Specifications
| Specification | Details |
|---|---|
| Product Name | Serum-Free and DMSO-Free Cell Freezing Medium |
| Catalog No. | FG-BA3341 |
| Pack Size | 100 mL |
| Formulation Features | Serum-free, animal-origin-free, DMSO-free, non-toxic |
| Compatible Samples | Animal primary cells and immortalized cell lines |
| Main Function | Cell cryopreservation and preservation |
| Workflow | Cell counting, centrifugation, resuspension in freezing medium, cryovial aliquoting, controlled-rate cooling, -80°C storage, optional liquid nitrogen transfer |
| Controlled-Rate Cooling | Recommended; approximately 1°C/min |
| General Cryopreservation Density | 1 × 10⁶ cells/mL |
| Storage After Freezing Cells | -80°C initially; transfer to liquid nitrogen after 24 hours for longer-term storage |
| Thawing Temperature | 37°C water bath |
| Example Thawing Centrifugation | 300 ×g for 5 minutes at 4°C |
| Required Instruments for Freezing | Horizontal centrifuge, cell counter, -80°C freezer |
| Required Instruments for Thawing | Horizontal centrifuge, water bath |
| Required Reagents for Thawing | RPMI 1640 medium or suitable culture medium |
| Required Consumables | Cell cryovials, programmable freezing container, low-adsorption pipette tips, centrifuge tubes, alcohol cotton balls |
| Product Storage | -20°C, protected from light |
| Shelf Life | One year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| Serum-Free and DMSO-Free Cell Freezing Medium | FG-BA3341 | 100 mL |