Description
FireGene Small Mammal Ovarian Dissociation Kit is designed for the isolation and preparation of cell suspensions from ovarian tissues of small mammals. The kit uses enzymatic digestion to gently and effectively disrupt extracellular matrix and release cells. Prepared single-cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays. The protocol includes digestion, filtration, washing, resuspension, and quality control, with digestion time adjusted according to tissue condition.
Background Information
Small mammal ovarian tissue is widely used in reproductive biology, developmental studies, fertility models, and disease research. A dedicated dissociation workflow supports preparation of cell suspensions for downstream single-cell and culture-based applications.
FireGene Small Mammal Ovarian Dissociation Kit for Single-Cell Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Small mammal reproductive biology
- Ovarian biology
- Fertility and follicle research
- Developmental biology
- Endocrine research
- Single-cell biology
- Cell culture assay development
- Preclinical disease models
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Small mammal ovarian single-cell suspension preparation
- Single-cell sequencing
- Cell culture
- Cell viability and counting
- Cell-based assays
- Tissue heterogeneity analysis
- Reproductive disease model research
Specifications
| Product Name | Small Mammal Ovarian Dissociation Kit |
| Brand | FireGene |
| Catalog No. | FG-BA3352 |
| Kit Size | 10 reactions |
| Sample Type | Ovarian tissue from small mammals |
| Compatible Samples | Fresh ovarian tissue |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Preparation of ovarian single-cell suspensions |
| Workflow | Enzymatic digestion, filtration, centrifugation, washing, resuspension, quality control |
| Downstream Applications | Single-cell sequencing, cell culture, cell-related assays |
| Digestion Conditions | 37°C for 0.5–2 hours |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | PBS, FBS, RPMI 1640 medium |
| Required Consumables | Low-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | -20°C |
| Shelf Life | Two years |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| SMODS (Small Mammal Ovarian Dissociation Solution) | FG-BA3352-A | 2 × 1.25 mL |
Produt FAQ
Q: What small mammal species is this kit validated for?
A: The kit is optimized for mouse and rat ovarian tissue. It is also compatible with other small mammal species — digestion time should be adjusted based on tissue density and mass for non-validated species.
Q: Can the kit be used with frozen ovarian tissue?
A: The kit is designed for fresh ovarian tissue. For frozen tissue, a nuclei isolation workflow (snRNA-seq) is recommended instead. Contact us for guidance on selecting the right protocol.
Q: Is red blood cell removal required?
A: Optional. For highly vascularized samples or blood-contaminated preparations, the Red Blood Cell Lysis Solution (Cat. No. FG-BA3311) can be used as an add-on step after dissociation. It is not included in the kit but is available separately.
Q: What downstream single-cell platforms is this kit compatible with?
A: Validated for 10x Genomics Chromium, BD Rhapsody, and Drop-seq. The single-cell suspension output meets the viability and concentration requirements for standard microfluidic capture workflows.
Q: Is bulk pricing available?
A: Yes. The kit is available in 10-reaction and 50-reaction formats. For larger quantities or custom packaging, submit an inquiry at the form below or email service@firegene.com.
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