Description
FireGene Spinal Cord Dissociation Kit is designed for the isolation and preparation of single-cell suspensions from spinal cord tissue. The kit uses enzyme-based digestion to gently and rapidly disrupt extracellular matrix and release cells. The workflow includes digestion, filtration, washing, debris removal, optional red blood cell lysis, and quality control. Prepared cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays.
Background Information
Spinal cord tissue can contain fragile neural and glial cell populations along with debris generated during dissociation. A workflow that combines enzymatic digestion with debris removal helps improve sample clarity before downstream cellular analysis.
FireGene Spinal Cord Dissociation Kit for Single-Cell Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Neuroscience research
- Spinal cord injury models
- Neuroinflammation studies
- Neurodegeneration research
- Glial cell biology
- Regenerative medicine
- Single-cell biology
- Cell culture assay development
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Spinal cord single-cell suspension preparation
- Single-cell sequencing
- Flow cytometry and cell sorting
- Primary neural cell culture
- Cell viability and counting
- Debris reduction after tissue dissociation
- Cell-type composition analysis
Specifications
| Product Name | Spinal Cord Dissociation Kit |
| Brand | FireGene |
| Catalog No. | FG-BA3334 |
| Kit Size | 10 reactions |
| Sample Type | Spinal cord tissue |
| Compatible Samples | Fresh spinal cord tissue |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Preparation of spinal cord single-cell suspensions |
| Workflow | Enzymatic digestion, filtration, centrifugation, debris removal, washing, optional RBC lysis, resuspension |
| Downstream Applications | Single-cell sequencing, cell culture, cell-related assays |
| Digestion Conditions | 37°C for 20–30 minutes |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | PBS, FBS, RPMI 1640 medium |
| Required Consumables | Low-adhesion pipette tips, centrifuge tubes, 20 μm and 70 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | Buffer A and Enzyme B: -20°C; Enzyme C and DRS: 4°C, protected from light |
| Shelf Life | Buffer A and Enzyme B: 2 years; Enzyme C and DRS: 1 year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| Buffer A | FG-BA3334-A | 21.6 mL |
| Enzyme B | FG-BA3334-B | 8 mL |
| Enzyme C | FG-BA3334-C | 100 μL |
| DRS (Spinal Cord Cell Debris Removal Solution) | FG-BA3308-D | 10 mL |