Product Overview
Overview
The FireGene Spleen Dissociation Kit is designed to produce high-viability single-cell suspensions from spleen tissue through a carefully optimized enzymatic protocol. It is ideal for immunological studies, single-cell sequencing, and immune profiling applications, offering exceptional cell recovery and integrity.
Background Information
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Driven by Clinical and Scientific Research Needs:
- Single-cell sequencing of spleen tissue is critical for understanding immune mechanisms and identifying functional cell subsets.
- Traditional methods often fail to preserve rare or sensitive immune populations.
- This kit supports:
- Detailed profiling of T cells, B cells, dendritic cells, and macrophages.
- Investigation of immune responses in sepsis, autoimmune disorders, and cancer.
- Discovery of immunological biomarkers and therapeutic targets for translational and clinical research.
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Background of Technological Development:
- Mechanical disruption can damage spleen cells and yield inconsistent results.
- FireGene improves performance with:
- Enzyme cocktails tailored to the spleen’s extracellular matrix.
- Optimization of incubation times, temperatures, and concentrations.
- High reproducibility and superior cell viability for downstream assays.
Detection Principle
- Based on a synergistic multi-enzyme digestion process:
- Spleen tissue is minced into small sections to enhance reagent access.
- Enzymes are added sequentially under controlled conditions.
- The digestion breaks down ECM structures and cellular junctions to release single cells.
- Final Outcome:
- A high-quality single-cell suspension suitable for scRNA-seq, flow cytometry, and immunophenotyping.
Specifications
| Applications | Single-cell sequencing, cell culture or other cell-related detections |
| Compatible Sample Types | Spleen tissue |
| Supported Instruments | Water bath, horizontal centrifuge, cell counter |
| Storage | -20 °C |
| Shelf-life | 24 months |
Kit Components
| Component | 10 Tests/Kit |
| SDS | 2 × 1.25 mL |