FireGene Trachea Dissociation Kit – Respiratory Cell Isolation

Overview

The FireGene Trachea Dissociation Kit is a specialized enzymatic solution for isolating high-quality single-cell suspensions from tracheal tissue. Designed to support respiratory research and single-cell applications, this kit offers exceptional cell recovery and viability, ideal for understanding airway biology and disease mechanisms.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Studying respiratory diseases like tracheitis, tracheal tumors, and airway inflammation requires understanding cell-level heterogeneity.
  • Traditional dissociation approaches often compromise rare or delicate cell types.
  • This kit enables:
    • Identification of epithelial cells, ciliated cells, immune cells, and more from tracheal samples.
    • Discovery of biomarkers and pathways related to inflammation and remodeling.
    • Support for targeted therapy development and precision medicine in respiratory health.

• Background of Technological Development:

  • Mechanical and chemical methods lack consistency and damage viable cells.
  • FireGene’s approach integrates:
    • Optimized enzymatic digestion tailored to tracheal matrix structure.
    • Adjusted protocols for reaction time, temperature, and reagent ratios.
    • Superior cell viability, efficiency, and reproducibility in tracheal tissue dissociation workflows.

Detection Principle

• Employs a multi-enzyme digestion strategy:
  • Trachea tissue is minced into small fragments.
  • A controlled sequence of enzyme application is used.
  • Enzymes gradually break down extracellular matrix and cell-cell junctions.
• Result:
  • A clean, viable single-cell suspension optimized for scRNA-seq, immune profiling, and respiratory disease modeling.

Specifications

Detection Principle