FireGene Tumor Dissociation Kit – Optimized for Tumor Microenvironment

Overview

The FireGene Tumor Dissociation Kit is optimized for the enzymatic dissociation of tumor tissue into high-viability single-cell suspensions. Tailored for downstream applications such as single-cell RNA sequencing and functional analysis, this kit supports researchers in profiling the complex tumor microenvironment.

Background Information

• Driven by Clinical and Scientific Research Needs:

  • Single-cell sequencing enables detailed insights into tumor heterogeneity and progression.
  • Traditional dissociation methods fail to preserve fragile or rare cell types within tumor tissues.
  • The kit facilitates:
    • Isolation of diverse tumor-associated cells such as malignant cells, stem-like cells, and immune cells.
    • Exploration of treatment resistance, tumor development, and immune infiltration.
    • Identification of biomarkers and spatial expression profiles for drug discovery and personalized medicine.

• Background of Technological Development:

  • Conventional mechanical and chemical techniques often compromise cell viability and reproducibility.
  • This kit uses:
    • Refined enzyme mixtures selected based on tumor extracellular matrix characteristics.
    • Optimized reaction parameters for precise, efficient dissociation.
    • A process that preserves cell diversity and yields high-quality single-cell suspensions consistently.

Detection Principle

• Implements a multi-step enzymatic digestion method:
  • Tumor samples are minced into small pieces.
  • Two-stage enzymatic reagents are added sequentially.
  • Digestion proceeds under carefully controlled temperature and timing.
  • Enzymes break down ECM components and intercellular adhesions.
• Final result:
  • A clean, viable single-cell suspension ready for downstream applications like scRNA-seq, cytometry, or organoid development.

Specifications

Kit Components