Description
FireGene Tumor Dissociation Kit Plus is designed for the isolation and preparation of single-cell suspensions from complex mammalian tumor tissues. The kit uses enzymes to gently and rapidly break down extracellular matrix and release cells. Prepared tumor cell suspensions can be used for single-cell sequencing, cell culture, and other cell-related assays. Digestion time can be adjusted based on species, tissue amount, and tumor condition, including samples with higher necrosis.
Background Information
Tumor tissues often contain diverse cell populations, dense extracellular matrix, stromal components, necrotic regions, and immune cell infiltration. A tumor-focused dissociation workflow helps prepare single-cell suspensions for studying tumor heterogeneity and the tumor microenvironment.
FireGene Tumor Dissociation Kit Plus for Single-Cell Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Cancer biology
- Tumor microenvironment research
- Cancer immunology
- Drug discovery and screening
- Translational oncology
- Tumor heterogeneity studies
- Single-cell biology
- Cell culture assay development
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Tumor single-cell suspension preparation
- Single-cell RNA sequencing
- Flow cytometry and cell sorting
- Primary tumor cell culture
- Cell viability and counting
- Tumor microenvironment profiling
- Drug response and biomarker studies
Specifications
| Product Name | Tumor Dissociation Kit (Plus) |
| Brand | FireGene |
| Catalog No. | FG-BA3349 |
| Kit Size | 10 reactions |
| Sample Type | Complex mammalian tumor tissues |
| Compatible Samples | Fresh tumor tissue |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Preparation of tumor tissue single-cell suspensions |
| Workflow | Enzymatic digestion, filtration, centrifugation, washing, resuspension, quality control |
| Downstream Applications | Single-cell sequencing, cell culture, cell-related assays |
| Digestion Conditions | 37°C for 10–40 minutes; longer for samples with high necrosis |
| Required Instruments | Water bath or hybridization oven, horizontal centrifuge |
| Required Reagents | PBS, FBS, RPMI 1640 medium |
| Required Consumables | Low-adhesion pipette tips, 5 mL and 15 mL centrifuge tubes, 70 μm cell strainers |
| Optional Related Reagent | Red Blood Cell Lysis Solution, Catalog No. BA3311 |
| Storage | -20°C |
| Shelf Life | Two years |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| TDSP (Tumor Dissociation Solution (Plus)) | FG-BA3349 | 30 mL |