Description
FireGene Universal PBMC Isolation Solution is designed for the isolation of mammalian peripheral blood lymphocytes using a sedimentation method. The workflow prepares high-viability, low-impurity lymphocyte single-cell suspensions suitable for flow cytometry analysis, cell culture, and other cell-related assays. The protocol uses anticoagulated blood, PBS dilution, gentle mixing with the isolation solution, low-speed centrifugation, optional red blood cell lysis, washing, and final resuspension for downstream use.
Background Information
Sedimentation-based PBMC isolation provides a practical workflow for mammalian peripheral blood samples where a simple preparation method is preferred. The method helps separate mononuclear cells from red blood cells before washing and downstream analysis.
FireGene Universal PBMC Isolation Solution for Sedimentation-Based Lymphocyte Preparation provides a practical workflow for sample preparation while supporting downstream applications that require reliable cell or nuclei suspension quality.
Research Areas
This product is suitable for research fields involving tissue processing, cellular analysis, and downstream molecular or cell-based workflows.
- Mammalian immunology
- Peripheral blood cell analysis
- Inflammation research
- Preclinical disease models
- Immune cell profiling
- Flow cytometry assay development
- Cell culture workflows
- Translational research
Key Applications
The prepared samples can be used in downstream workflows that require clean, well-prepared cell or nuclei suspensions.
- Mammalian PBMC isolation
- Peripheral blood lymphocyte preparation
- Flow cytometry analysis
- Cell culture
- Cell-based assays
- Sample preparation from anticoagulated blood
- Immune cell profiling
Specifications
| Product Name | Universal PBMC Isolation Solution (Sedimentation Method) |
| Brand | FireGene |
| Catalog No. | FG-BA3344 |
| Pack Size | 100 mL |
| Sample Type | Mammalian peripheral blood |
| Compatible Samples | Anticoagulated blood |
| Main Function | Isolation of mammalian peripheral blood lymphocytes |
| Workflow | Blood dilution, sedimentation-based separation, centrifugation, supernatant collection, optional RBC lysis, washing, resuspension |
| Downstream Applications | Flow cytometry analysis, cell culture, cell-related assays |
| Centrifugation for Separation | 20°C, 200 ×g, 7 minutes |
| Required Instruments | Horizontal centrifuge, cell counter |
| Required Reagents | PBS, FBS |
| Required Consumables | Low-adsorption pipette tips, 15 mL centrifuge tubes |
| Optional Related Reagent | Red Blood Cell Lysis Buffer, Catalog No. BA3311 |
| Storage | 4°C, protected from light |
| Shelf Life | One year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| Universal PBMC Isolation Solution | FG-BA3344 | 100 mL |