Description
FireGene Blood Vessel Nuclei Isolation Kit is designed for the isolation and extraction of nuclei from cell suspensions, fresh tissues, and cryopreserved tissues derived from animal vascular tissues. The kit uses surfactants to disrupt cell membranes and release nuclei while maintaining nuclear membrane integrity. After tissue grinding, filtration, centrifugation, and density-layer separation, the workflow produces clean single-nucleus suspensions suitable for single-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, and related nuclear analysis experiments.
Background Information
Blood vessels contain multiple specialized cell populations, including endothelial cells, smooth muscle cells, pericytes, fibroblasts, and immune-associated cells. Preparing nuclei from vascular tissue can support molecular profiling when intact cell dissociation is challenging or when cryopreserved tissue samples are used.
FireGene Blood Vessel Nuclei Isolation Kit provides a practical workflow for preparing clean single-nucleus suspensions from animal vascular samples for sequencing and nuclear analysis.
Research Areas
This kit is suitable for research fields focused on vascular structure, gene regulation, and tissue-level cellular diversity.
- Vascular biology
- Cardiovascular disease research
- Endothelial cell research
- Smooth muscle cell biology
- Atherosclerosis and vascular inflammation studies
- Angiogenesis research
- Tissue remodeling and fibrosis research
- Single-cell and single-nucleus multiomics
- Translational biomedical research
Key Applications
The prepared nuclei suspensions can be used in downstream workflows requiring clean nuclear material.
- Single-nucleus RNA sequencing, or snRNA-seq
- Single-nucleus ATAC sequencing, or snATAC-seq
- Nuclear transcriptome analysis
- Chromatin accessibility profiling
- Cell-type composition analysis in vascular tissue
- Gene expression profiling from fresh or cryopreserved vascular samples
- Vascular tissue heterogeneity studies
- Disease mechanism research in cardiovascular models
Specifications
| Specification | Details |
|---|---|
| Product Name | Blood Vessel Nuclei Isolation Kit |
| Brand | FireGene |
| Kit Size | 10 reactions |
| Sample Source | Animal vascular tissues |
| Compatible Samples | Cell suspensions, fresh tissues, and cryopreserved tissues derived from animal vascular tissues |
| Recommended Starting Material | Approximately 200 mg tissue |
| Main Function | Isolation and extraction of nuclei |
| Workflow | Liquid nitrogen grinding, lysis, filtration, centrifugation, density-layer separation, nuclei washing and resuspension |
| Downstream Applications | Single-nucleus transcriptome sequencing, single-nucleus ATAC sequencing, related nuclear analysis experiments |
| Required Instruments | Horizontal centrifuge, tissue grinder, cell counter |
| Required Tissue Tools | Surgical scissors, scalpels, forceps |
| Required Reagents | RNase inhibitor for nuclear RNA-related experiments |
| Required Consumables | Liquid nitrogen, grinding tubes with zirconium beads, 5 mL and 15 mL centrifuge tubes, 40 μm cell strainers, optional 20 μm cell strainers |
| Processing Temperature | Post-lysis steps should be performed on wet ice or an ice block when possible |
| Storage | Main reagents: 4°C, protected from light |
| Additive Storage | Additive Solution ① and Additive Solution ②: -20°C, protected from light |
| Shelf Life | One year |
| Research Use | For research use only |
Kit Components
| Component | Catalog Number | Pack Size |
|---|---|---|
| Lysis Buffer | FG-BA3343-A | 10 mL |
| Pre-Suspension Buffer | FG-BA3343-B | 15 mL |
| Post-Suspension Buffer | FG-BA3343-C | 50 mL |
| PB 1 | FG-BA3343-D | 3 mL |
| PB 2 | FG-BA3343-E | 6 mL |
| PB 3 | FG-BA3343-F | 6 mL |
| Additive Solution ① | FG-BA3343-G | 4 mL |
| Additive Solution ② | FG-BA3343-H | 150 μL |