Description
Product Introduction
There is no need to waste precious samples by re-running gels and electrophoresis to test different primary antibodies; simply treat the membrane with this antibody stripping buffer to remove the initial primary antibody. This step typically takes 10–20 minutes, depending on the amount of primary antibody bound to the membrane. Following stripping, the membrane is ready for re-probing with a new primary antibody.
Product Features
Save samples：The same sample on a single membrane can be used for multiple assays;
Saves time：No need to recast the electrophoresis gel;
Highly efficient and rapid：It offers excellent removal performance, far superior to homemade buffers;
Gentle formula: There is minimal damage to the target proteins bound to the transfer membrane;
Odorless：No pungent odor; more eco-friendly.
Instructions for Use
1. After completing the Western blot chemiluminescence detection, wash the membrane with TBS/T or PBS/T for 5 minutes;
2. Add 10–20 mL of stripping buffer to cover the membrane. Rinse on a shaker for 10–20 minutes. The stripping time depends on the amount of antibody bound to the membrane;
3. Discard the stripping buffer and rinse the membrane with TBS/T or PBS/T for 5 minutes. At this stage, the chemiluminescent substrate can be applied to the membrane to quickly verify the effectiveness of the antibody stripping;
4. Re-block the membrane with an appropriate blocking solution, and it can be re-probed with other antibodies;
5. Using this stripping buffer, the membrane can be stripped at least 10 times without significantly affecting signal intensity. Experiments using GAPDH as an example demonstrated that the GAPDH signal did not decrease significantly after 6 hours of continuous stripping—equivalent to 24 stripping cycles (based on 15 minutes per cycle).
Precautions
1. Reuse of this product is not recommended;
2. The transfer film will turn transparent upon contact with the release solution; this is normal, so please use it with confidence;
3. For your safety and health, please wear a lab coat and disposable gloves while performing operations.
4. This product is for research use only.