Reverse Transcription and cDNA Synthesis
The RT step sits between your RNA and everything downstream — and it's where more variation enters the workflow than most protocols acknowledge. Template secondary structure, genomic DNA contamination, inhibitor carryover from extraction, primer bias: each of these affects cDNA yield and representation before the first qPCR cycle runs.
This collection covers five products across three RT workflow formats, each addressing a different combination of input and downstream application.
Standalone reverse transcriptase MagicScript II Reverse Transcriptase for labs that run their own optimized RT protocols — high thermostability for GC-rich or structured templates, compatible with oligo(dT), random hexamers, or gene-specific primers.
First-strand cDNA synthesis kits Two formats: standard First Strand cDNA Synthesis Kit for clean RNA inputs, and a gDNA Wiper variant that removes genomic DNA contamination in the same tube before reverse transcription begins. No separate DNase incubation step, no additional reagent, no extra time — the wiper reagent is added first, incubated briefly, then the RT reaction proceeds in the same tube.
RT mixes for qPCR Two RT Mix for qPCR formats — standard and gDNA Wiper — using a balanced random primer/oligo(dT) ratio for uniform coverage across transcript lengths. Optimized for two-step RT-qPCR where the cDNA is used directly in downstream qPCR without cleanup.
