Overview
The FireGene Dead Cell Debris and Impurities Removal Kit is designed to purify single-cell suspensions by removing dead cells, apoptotic bodies, and unwanted debris. This enhances the clarity, viability, and analytical accuracy of your cell samples, especially in applications like flow cytometry, single-cell sequencing, and immunocytochemistry.
- Used extensively to improve sample quality for downstream applications.
- Benefits include:
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Improved cell sorting and gating accuracy in flow cytometry.
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Enhanced data quality in scRNA-seq and proteomics.
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Increased reliability of results in cell culture by enriching live cell populations.
- Valuable in immunocytochemistry and staining assays, where background noise from dead cells must be minimized.
Detection Principle
- Operates via density gradient centrifugation:
- Cell suspensions are layered and spun under controlled conditions.
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Low-density dead cells and debris float in the upper layer.
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Viable, intact cells sink to the bottom.
- Final output:
- A clean, enriched single-cell suspension optimized for high-precision biological research.
Specifications
| Applications |
Single-cell sequencing, cell culture or other cell-related detections |
| Compatible Sample Types |
Single-cell suspension |
| Supported Instruments |
Water bath, horizontal centrifuge, cell counter |
| Storage |
4 °C |
| Shelf-life |
12 months |
Kit Components
| Component |
10 mL/Kit |
20 mL/Kit |
| DRS |
10 mL |
20 ml |
